摘要
利用高速逆流色谱技术分离纯化白鲜皮中的梣酮,结果表明,选择V(正己烷):V(乙酸乙酯):V(乙醇):V(水)=1:1:1:1系统来分离,上相作为固定相,下相作为流动相,流速为1.5 mL/min,仪器转速1 000 r/min,分离结果经高效液相色谱(HPLC)检测纯度达到99%。
High-speed Countercurrent Chromatography（HSCCC） was used to isolate and purify Fraxinellone from Dictamnus dasycarpus.Isolation was performed with a two-phase solvent system composed of hexane：ethylacetate：ethanol：water = 1：1：1：1：1（V/V）by eluting the lower aqueous phase at a flow-rate of 1.5 mL/min and a revolution speed of 1 000 r/min.The purified Fraxinellone could reach purity of 99% by high performance liquid chromatograph（HPLC）.HSCCC is recommended to prepare and purify the active constituents from D.dasycarpus.
出处
《浙江林业科技》
北大核心
2010年第6期52-54,共3页
Journal of Zhejiang Forestry Science and Technology
关键词
白鲜皮
高速逆流色谱
分离
梣酮
Dictamnus dasycarpus
High-speed Countercurrent Chromatography
isolation
Fraxinellone
