摘要
为了得到溶藻物质并进行溶藻特性的研究,采用离心、乙醇沉淀和有机溶剂萃取的方法从发酵液中粗提得到溶藻物质,再通过硅胶吸附柱层析和透析进行精制,并采用试管试验法对其进行分析。研究溶藻物质的pH稳定性、热稳定性以及对藻类抗氧化酶系统的影响。该菌株的溶藻物质具有较大极性,且分子量小于3 kD;经化学法鉴定,基本确定为糖苷类物质、内酯类物质和萜类物质中的一种或几种的组合;具酸碱稳定性,但对热敏感,随着处理温度的升高,溶藻效果逐渐下降,当温度超过90°C处理1 h后,活性几乎全部丧失;会使藻细胞的丙二醛含量明显上升且维持在较高水平,抗氧化酶系统中的SOD、POD和CAT在初期明显上升,但在后期剧烈下降。
To obtain the algicidal components from actinomycete strain L74,a series of techniques in-cluding centrifugation,ethanol precipitation,organic solvents extraction,silica gel adsorption chromatog-raphy,dialysis and chemical analysis were used.In addition,we studied the characteristic of stabilities of algicidal components under different pH and temperatures,and the influence of it to anti-oxidation en-zyme system of Microcstis aeruginosa.The results suggested that the algicidal components may be or contain glycosides,lactones and triterpenes,which the molecular weights are less than 3.0 kD.In extrac-tion experiments,petroleum ether,acetic ether and n-butanol can efficiently separate the algicidal com-ponents into water-layer.The algicidal components are stable for pH,but sensitive for temperature.After treated under 90 °C for one hour,the activity is nearly lost.When cultivated with algicidal components,the MDA contents of Microcstis aeruginosa increase and maintain at a high level,but the activities of SOD,POD and CAT significantly increase in the early cultivation and decline quickly in the late stage.
出处
《微生物学通报》
CAS
CSCD
北大核心
2011年第5期654-659,共6页
Microbiology China
基金
国家自然科学基金项目(No.21076090)
关键词
溶藻物质
分离
稳定性
抗氧化酶
Algicidal components
Isolation
Stability
Anti-oxidation enzyme system
