摘要
在对溶藻细菌L7所分泌的溶藻活性物质进行分离和纯化后,通过质谱法、紫外光谱扫描法、红外光谱扫描法以及特征显色法对该活性物质进行了初步鉴定.结果表明,质谱分析出第12#组分中的5种物质分子量分别为243.7,364.0,508.1,602.1,678.7.紫外光谱扫描分析出溶藻细菌L7溶藻活性代谢产物可能含有2个共轭的不饱和键.红外光谱扫描和薄层层析特征显色结果显示,其可能含有芳香醛类、氨基酸类、醇类或糖类物质,但一定不含有酚类物质.
Following the isolation and purification of active compounds secreted by algicidal bacteria L7, the analytical methods of L7, LC-MS, UV, IR and TLC were applied to identify the active compounds .The LC-MS showed that the 12~# component contains five compounds, whose molecular weights are 243.7, 364.0, 508.1, 602.1, 678.7, respectively. The results of UV indicated that the structure of algicidal compounds might contain two conjugated and unsaturated bonds. The results of IR and TLC indicated that the active compounds might contain aromatic aldehyde, amino acid, alcohol or the saccharide, but not phenol.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2010年第S1期19-23,共5页
China Environmental Science
关键词
溶藻细菌
溶藻活性物质
分离
鉴定
algicidal bacteria
extracellular algae-lysing constituents
isolation
identification
