摘要
通过对阿奇霉素显色反应过程中硫酸浓度,反应时间,反应温度的研究,优化了分光光度法测定阿奇霉素含量的方法。结果表明,阿奇霉素在11.26—45.03mg.L-1浓度范围内,显色溶液在482nm处吸光度与其浓度具有良好的线性关系,线性回归方程为A=0.0172C(mg.L-1)+0.0504,平均回收率为99.1%(n=9)。该方法简单,比传统光度法更灵敏、准确,适合于阿奇霉素含量的快速测定。
The spectrophotometric method for determination of azithromycin based on the color reaction of sulfuric acid and azithromycin was optimized and all variables reaction conditions such as sulfuric acid concentration,heating time,temperature were carefully studied.The result showed the maximum absorbance at 482nm is linear to concentration of azithromycin with A=0.0172C（mg·L-1）＋0.0504 in the range of 11.26—45.03mg·L-1.The average recovery was 99.1%（n=9）.This optimized method is simple,sensitive,accurate,and suitable for the rapid determination of azithromycin.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第3期1499-1502,共4页
Chinese Journal of Spectroscopy Laboratory
关键词
阿奇霉素
分光光度法
硫酸
显色反应
Azithromycin
Spectrophotometry
Sulfuric Acid
Color Reaction
