摘要
采用荧光光谱技术研究了胡椒碱与牛血清白蛋白(BSA)的相互作用。根据测定不同温度下胡椒碱对BSA的猝灭常数,证实了荧光猝灭过程为静态猝灭,由热力学参数焓变(ΔH)小于零和熵变(ΔS)大于零,推断出胡椒碱与BSA之间主要靠静电引力相结合,生成自由能变(ΔG)为负值,表明胡椒碱与BSA的作用过程是一个自发过程;并应用同步荧光光谱技术考察了胡椒碱对BSA构象的影响。
The interaction between piperine and bovine serum albumin（BSA） was studied by fluorescence spectroscopy.The quenching constants were obtained at different temperatures,the results indicated that the fluorescence quenching mechanism for BSA through piperine binding was likely a static quenching process.The thermodynamic parameters,enthalpy change ΔH0 and entropy change ΔS0,which indicated that the interaction of piperine with BSA was driven mainly by electrostatic force.The process of binding was a spontaneous process in which Gibbs free energy change ΔG0.The effect of piperine on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第3期1236-1238,共3页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金(20975081)
西北大学研究生交叉学科资助项目(07YJC09)
陕西省教育厅科研计划项目(2010JK899)
咸阳师范学院专项科研基金(10XSYK205)
关键词
胡椒碱
牛血清白蛋白
荧光光谱
Piperine
Bovine Serum Albumin
Fluorescence Spectroscopy
