摘要
采用荧光光谱技术研究了橙皮素与牛血清白蛋白(BSA)的相互作用。研究发现橙皮素对BSA的荧光猝灭属于静态猝灭过程,由热力学参数焓变△rHm=-60.543 kJ.mol-1,熵变△rSm=-114.121J.mol-1均小于零,判断橙皮素与BSA之间主要靠氢键和范德华力相结合,生成自由能变(△rGm)为负值,表明橙皮素与BSA的作用过程是一个自发过程;并应用同步荧光光谱考察了橙皮素对BSA构象的影响。
The interaction behavior between Hesperetin and Bovine Serum Albumin（BSA） was studied by fluorescence spectroscopy.The quenching constants were obtained at 292K,299 K,311K and 315t K,the results indicated that the fluorescence quenching mechanism for BSA through Hesperetin binding is likely a static quenching process.The thermodynamic parameters,enthalpy change（△rHm） and entropy change（△rSm）,were calculated to be-60.543 kJ·mol-10,and-114.121J·mol-10,respectively,which indicated that the interaction of Hesperetin with BSA was driven mainly by hydrogen bond and Van der Walls force.The process of binding was a spontaneous process in which Gibbs free energy change（△rGm） was negative.The effect of Hesperetin on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.
出处
《化学工程师》
CAS
2011年第1期12-13,17,共3页
Chemical Engineer
基金
国家自然科学基金项目(20975081)
陕西省教育厅科研计划项目(2010JK899)
西北大学研究生交叉学科资助项目(07YJC09)
关键词
橙皮素
牛血清白蛋白
荧光光谱
Hesperetin
Bovine Serum Albumin（BSA）
fluorescence spectroscopy
