摘要
目的了解Hedgehog信号通路在乳腺癌发生发展中的作用。方法采用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD44+CD24-细胞和非CD44+CD24-细胞,分别接种于NOD/SCID鼠乳腺脂肪垫内,观察成瘤情况。采用real-time RT-PCR技术检测Hedgehog信号通路分子SMO和GLI1在CD44+CD24-细胞和非CD44+CD24-细胞中的表达情况。结果分选出的CD44+CD24-细胞约占细胞总数的2.25%,SMO mRNA和GLI1 mRNA在CD44+CD24-细胞中的表达均高于其在非CD44+CD24-细胞中的表达(P均<0.05)。CD44+CD24-组乳腺癌发生率为100%(3/3),发生肺转移、淋巴结和肺转移、肝脏和淋巴结转移各1只;非CD44+CD24-细胞组乳腺癌发生率为50%(2/4),未见有转移发生。结论在乳腺癌CD44+CD24-细胞中Hedgehog信号通路分子表达增强,其与乳腺癌的浸润转移关系密切。
Objective To explore the effect of Hedgehog signal pathway in the progress of breast cancer.Methods CD44＋CD24- cells and non-CD44＋CD24- cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system（MACS） and injected in NOD/SCID mice,respectively.Real-time RT-PCR was employed to detect the expression of Hedgehog signal molecule SMO and GLI1 mRNA in CD44＋CD24- cells and non-CD44＋CD24- cells,respectively.Results CD44＋CD24- cells accounted for 2.25% of breast cancer cells.The expression levels of SMO and GLI1 mRNA in CD44＋CD24- cells were higher than those in non-CD44＋CD24- cells（P〈0.05）.The rate of tumor formation was 100%（3/3） in CD44＋CD24- cells contrast to 50%（2/4） in non-CD44＋CD24- cells.One mouse had lung metastasis,one mouse had lymph node and lung metastasis,and one mouse had liver and lymph node metastasis in CD44＋CD24- mice,while no organ metastasis was displayed in non-CD44＋CD24- mice.Conclusion Hedgehog signal pathway is activated in breast cancer CD44＋CD24- cells and plays an important role in breast carcinogenesis.
出处
《山东医药》
CAS
北大核心
2011年第16期33-35,118,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(30870968)
教育部高等学校博士学科点专项科研基金(200801610001)
