摘要
目的了解Hedgehog信号通路在乳腺癌发生发展中的作用。方法用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD44+CD24-细胞和非CD44+CD24-细胞,用real-time RT-PCR法检测Hedgehog信号通路主要分子SHH、PTCH1、SMO和GLI1 mRNA在细胞中的表达,用免疫组织化学法检测上述因子在乳腺癌组织中的表达。结果分选出的CD44+CD24-细胞约占乳腺癌悬浮细胞总数的8.25%,分选出的CD44+CD24-细胞表达干细胞标志蛋白ALDHA1和Oct-4;SHH、PTCH1、SMO和GLI1 mRNA在CD44+CD24-细胞中的表达均高于其在非CD44+CD24-细胞中的表达(P<0.05);SMO和GLI1蛋白在三阴性乳腺癌的表达均高于非三阴性乳腺癌组织(P<0.05)。结论在乳腺癌干细胞CD44+CD24-细胞中Hedgehog信号通路被激活,抑制癌症干细胞中Hedgehog通路的活化可能会降低或阻止乳腺癌的复发及化疗耐受。
Objective To explore the effect of Hedgehog signaling pathway in breast carcinogenesis.MethodsCD44＋CD24-cells and non-CD44＋CD24-cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system（MACS）,RT-PCR was employed to detect the expression of SHH,PTCH1,SMO and GLI1 mRNA in CD44＋CD24-cells and non-CD44＋CD24-cells,immunohistochemistry test was applied to study the expressions of SHH,PTCH1,SMO and GLI1 protein in breast cancer.ResultsCD44＋CD24-cells accounted for 8.25% of breast cancer suspension cells and showed positive expression of ALDH1A1 protein and Oct-4 protein.The expression of SHH,PTCH1,SMO and GLI1 mRNA in CD44＋CD24-cells were higher than those in non-CD44＋CD24-cells.SMO and GLI1 expressions were increased in triple-negative breast cancer.Conclusion Hedgehog signaling is activated in breast cancer stem cell CD44＋CD24-cells and so to prevent the recurrence and drug resistance through inhibiting the activation of Hedgehog signaling in breast cancer cells may be an effective measure.
出处
《基础医学与临床》
CSCD
北大核心
2011年第4期383-387,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(30870968)
教育部高等学校博士学科点专项科研基金(200801610001)
