摘要
通过建立过氧化氢(H2O2)对C2C12细胞的氧化损伤模型,从细胞水平探讨β-酪啡肽-5(β-casomorphin5,β-CM5)对H2O2致氧化应激损伤的保护作用及其可能机制。方法:1)MTT法测定72h内β-CM5对C2C12细胞活力的影响,筛选安全剂量;2)测定不同浓度H2O2对C2C12细胞活力的影响,筛选H2O2的最佳剂量和作用时间;3)测定β-CM5对H2O2损伤后细胞活力的影响;4)检测培养液中超氧化物歧化酶(superoxidedismutase,SOD)、乳酸脱氢酶(lactate dehydrogenase,LDH)的活性。结果:1)β-CM5各浓度组MTT值与对照组无差异;2)0.25mmol/L的H2O2作用于C2C12细胞1h,MTT值显著低于对照组(P<0.01);3)与损伤组相比,β-CM5预保护组的MTT值显著升高(P<0.05),治疗组MTT值无明显变化;4)与损伤组相比,β-CM5预保护组的SOD活性显著提高(P<0.05),LDH活性显著下降(P<0.05)。结论:β-CM5具有较强的抗氧化作用,对H2O2损伤的C2C12细胞有明显的保护作用,其机制可能与增强抗氧化酶活力和维持细胞完整性有关。
Objective： To explore the protective effect and potential mechanism of β-casomorphin5（β-CM5） against hydrogen peroxide（H2O2）-induced oxidative damage in C2C12 cells.Methods： MTT method was used to determine the effect of β-CM5 on cell viability within 72 h and to screen the safe dose.Meanwhile,the effect of H2O2 with various concentrations on cell viability was also evaluated.In addition,the activities of superoxidedismutase（SOD） and lactate dehydrogenase（LDH） in medium were determined.Results： Compared with the control group,the values of MTT in β-CM5 groups with various concentrations did not exhibit an obvious difference.In contrast,a significant decrease in MTT values of C2C12 cells was observed after 1 h of treatment with 0.25 mmol/L H2O2（P 0.01）;Compared with the oxidative damage group,MTT value in the β-CM5 pre-protection group was significantly higher（P 0.05）,while MTT value in the β-CM5 treatment group had no significant difference when compared to the oxidative damage group.Compared with the oxidative damage group,the activity of SOD in β-CM5 pre-protection group was significantly increased（P 0.05）,while the activity of LDH was significantly decreased（P0.05）.Conclusion：β-CM5 has a strong antioxidant and pre-protective effect on H2O2-induced damage of C2C12 cells.The mechanism may be related to the increased activity of antioxidant enzymes and maintenance of cell integrality.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第9期257-260,共4页
Food Science
基金
国家自然科学基金项目(300070565)
