摘要
目的利用现代生物技术方法生物合成红景天苷,探索提高红景天苷含量的生物转化最佳条件,为工业化大生产提供科学依据。方法以转基因西洋参冠瘿组织为培养体系,生物转化底物酪醇;利用HPLC对转化产物红景天苷进行含量测定;考察不同培养条件对西洋参冠瘿组织生长量及其转化产物红景天苷的影响。结果在摇床培养中西洋参冠瘿组织培养基为"MS+蔗糖"中红景天苷转化率达到49.50%;"1/2MS+蔗糖"培养基的转化率为49.98%;"MS+蔗糖+0.5 mg.L-1VB6"培养基的转化率达55.54%;此外,还利用生物反应器对西洋参冠瘿组织生物转化红景天苷进行了初步研究,转化率为33.42%。结论本实验通过考察不同培养条件对西洋参冠瘿组织生物合成红景天苷的影响,提高了红景天苷的转化率,并在摇瓶培养的基础上,初步实现了生物反应器对西洋参冠瘿组织生物合成红景天苷的生产,从而为该产品的工业化生产提供科学资料。
OBJECTIVE To seek for the optimal biotransformation condition for increasing the yield of salidroside,thus to provide valuable information for its industrial production on large scale.METHODS Tyrosol was used as substrate.The yield of salidroside was measured by HPLC.The influence of culture conditions on the grows of Panax quinquefolium crown galls and the salidroside yield were investigated.RESULTS MS medium was the best for salidroside accumulation（49.50%） among the 6 media.VB6 in concentration of 0.5 mg·L-1 could obviously promote salidroside biosynthesis（55.54%）.CONCLUSION It is the first study to biosynthesize tyrosol using P.quinquefolium crown galls.The biotransformation rate of salidroside in airlift bioreactor is increased.The information will be valuable for the industrial production of salidroside by biotechnological method in the future.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第9期656-660,共5页
Chinese Pharmaceutical Journal
关键词
西洋参冠瘿组织
生物转化
红景天苷
转化率
Panax quinquefolium crown galls
biotransformation
salidroside
transfer rate
