摘要
对西洋参冠瘿组织悬浮培养生长特征进行了考察,并对其悬浮培养物中的人参皂苷类成分进行了提取、分离和鉴定。研究得到了培养物最大生物量收获时间[18.62gL(dryweight)]及其中最高人参皂苷累积时间(620.4mgLonthe27thday)。培养基中碳源、磷、氨基氮、硝基氮的利用率分别为91.8%,100%,81%和97%。利用现代分离纯化方法从培养物中分离得到了4种人参皂苷类成分,利用理化及谱学技术分别鉴定为假人参皂苷F11(pseudoginsenosideF11,Ⅰ),人参皂苷Rd(ginsenosideRd,Ⅱ),人参皂苷Rb1(ginsenosideRb1,Ⅲ)和人参皂苷Rb3(ginsenosideRb3,Ⅳ)。
The growth characteristics and ginsenosides isolation of the suspension-cultured crown gall of Panax quinquefolium were studied. The result showed that the maximum biomass of cultures was 18.6g/L（dry weight） and the content of ginsenosides reached its maximum level of 620.4 mg/L on the 27th day. The utilization rates of sugar, phosphorus, nitrogen in NH4 ＋ and nitrogen in NO3- were 91.8%, 100%, 81% and 97%, respectively. Four compounds were isolated from the suspensioncultured crown gall and their structures were elucidated as pseudoginsenoside F11 （ Ⅰ ）, ginsenoside Rd （ Ⅱ ）, ginsenoside Rb1（ Ⅲ ） and ginsenoside Rb3 （ Ⅳ ）.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第5期754-758,共5页
Chinese Journal of Biotechnology
基金
国家教育部科学技术研究重点项目基金(No.104180)
广东省自然科学基金(No.31891)
广东省中医药局科研计划项目基金(No.103041)资助。~~
关键词
西洋参
冠瘿组织
悬浮培养
人参皂苷
分离
crown gall, ginsenosides, isolation, Panax quinquefolium, suspension cultures
