摘要
目的探讨肺炎支原体(MP)DNA及MP抗体联合检测的临床诊断价值。方法收集临床疑似MP感染的268例患儿样本,凝集法检测血清MP-抗体、实时荧光定量PCR法检测MP-DNA。结果 268例病例中65例确诊为MP肺炎,两种方法灵敏度分别为70.77%、87.69%,差异有统计学意义(P<0.05);特异度分别为95.07%、96.06%,差异无统计学意义。结论对疑似MP感染患儿同时进行抗体和DNA检测,可有效避免漏检。
OBJECTIVE To evaluate the methods for detection and diagnosis of mycoplasma infections in children with pneumonia.METHODS A total of 268 samples were collected from the patients with MP,and mp-antibody was detected by using passive agglutination（PA） method,MP-DNA loads was examined by real-time PCR.RESULTS Of 268 patients with pneumoniae,68 children were confirmed to be positive of Mycoplasma pneumoniae.The positive rate of detection was significantly higher with PCR than with antibody（P0.05）.There was no significant difference between the specificity of the two methods（P0.05）.CONCLUSION PCR method and PA are highly applicable in clinical laboratories for the preliminary detection and diagnosis of mycoplasma infections.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第10期2135-2136,共2页
Chinese Journal of Nosocomiology
基金
贵州省卫生厅科学技术基金项目(GZWKJ2008-1-021)
关键词
肺炎支原体
聚合酶链式反应
被动凝集试验
Mycoplasma pneumoniae
Polymerase chain reaction
Passive agglutination
