摘要
目的以肺炎支原体(Mp)重组蛋白做包被抗原检测临床呼吸道感染患儿血清标本,验证重组蛋白抗原的诊断价值。方法利用Mp重组蛋白抗原包被酶标板,ELISA法检测临床疑似Mp感染患儿的血清标本,同时用Mp膜抗原被动凝集试验平行检测,并对比分析两种方法的检测结果。结果重组蛋白抗原ELISA检测Mp抗体的阳性率为49.00%(98/200),膜抗原被动凝集试验Mp抗体的阳性率为63.50%(127/200)。两种检测方法的总符合率为70.50%。阳性符合率为58.45%。结论Mp重组蛋白抗原ELISA检测肺炎支原体感染有诊断价值,其特异性优于膜抗原被动凝集试验。
Objective Recombinant protein of Mycoplasma pneumoniae（Mp） was used as a coating antigen to detect Mp in serum samples from children who probably had clinical respiratory infections.The results were compared and analyzed to verify the diagnostic value of recombinant protein antigen.Methods Recombinant protein was coated on ELISA plates to detect clinical cases of suspected Mp infection using serum samples from children.At the same time,serum samples were subjected to Mp membrane antigen commercial reagent in a passive agglutination test. Results of these two methods were used for further comparison and analysis. Results In ELISA with Mp recombinant protein antigen, Mp antibody was positive in 49.00% of samples（98/200） ; in an Mp passive agglutination test with Mp membrane antigen, Mp antibody was positive in 63. 50% of samples（127/200）. The total rate of concordance for the two methods was 70.5%. The rate of concordance for a positive reaction was 58.45%. Conclusion ELISA with recombination protein antigen proved helpful in diagnosing Mp infection, and use of recombination protein antigen provided specificity superior to membrane protein antigen.
出处
《中国病原生物学杂志》
CSCD
2009年第11期804-805,826,共3页
Journal of Pathogen Biology
基金
辽宁省教育厅基金项目(No.2004D226)
关键词
肺炎支原体
重组蛋白抗原
膜蛋白抗原
ELISA
Mycoplasma pneumoniae（Mp）
recombination protein antigen
membrane protein antigen
ELISA
