摘要
目的 通过观察p53、bcl-2在泪腺良、恶性肿瘤中表达情况,分析其与泪腺良、恶性肿瘤的临床和组织病理的关系.目的在于进一步探明它们在泪腺良、恶性肿瘤发生、发展中的作用,为泪腺良、恶性肿瘤的鉴别诊断、治疗以及预后判断提供依据.方法 收集手术切除35例泪腺多形性腺瘤,28例泪腺腺样囊性癌,并取手术中切除的正常泪腺组织9例作为正常对照.应用免疫组化检测p53和bcl-2的表达,检验其在上述组织中表达的差异.结果 p53抗原、bcl-2抗原在正常泪腺及泪腺良、恶性肿瘤中的表达指数依次增高.p53、bcl-2在复发组中的表达高于初发组.72例标本中,p53和bcl-2两项均为阳性者为26例,均为阴性者22例,与单项阳性者相比具有显著性差异.结论 p53、bcl-2可以作为预测泪腺多形性腺瘤术后复发的有效检测指标.p53、bcl-2可能参与了泪腺肿瘤的发生、发展,将可能作为一种鉴别正常泪腺组织、泪腺良性肿瘤组及恶性肿瘤的有效的生物学指标.p53和bcl-2之间的相关性提示泪腺肿瘤的发生是一个多阶段、多因素、长期的过程.
Objective To investigate the expression of p53 and bcl-2 in normal lacrimal, benign and malignant neoplasms of lacrimal gland, to analyze relationship between above biological remarks and clinical or pathological of lacrimal tumors, to study their action in occurrence of lacriaml benign, malignant tumor and to provide evidence for differential diagnosis, therapy and prognosis estimation. Method Of 35 Pleomorphic adenoma, 28 Malignant lacrimal gland tumors and 9 normal lacrimal gland specimens were collected. The expression of p53 and bcl-2 was analyzed with immunohistochemistry in order to find the difference in the above-mentioned tissues. Results The expression of p53 and bcl-2 shows an increasing tendency from normal lacrimal to benign and malignant. Both p53 and bcl-2 expression were positive in 26 cases. Neither p53 nor bcl-2 was positive in 22 cases. These numbers were significantly higher than that single positive or monomial negative （P 〈0.05）. Conclusions p53 and bcl-2 may involve occurrence of lacrimal epithelial tumors and may be a biological remark of identifying normal lacrimal gland, lacrimal benign or malignant tumor. p53 and bcl-2seems to participate in occurring of lacrimal malignant tumors and be biological parameters which identify malignant from benign lacrimal gland. This study indicates that p53 and bcl-2 play important roles in lacrimal gland epithelial neoplasms. They are co-operative factors in the process of occurrence and development of lacrimal gland adenoid cystic carcinoma.
出处
《中国实用眼科杂志》
CSCD
北大核心
2011年第4期407-411,共5页
Chinese Journal of Practical Ophthalmology
