摘要
液相芯片技术是一种利用混悬在液相中的分类编码微球作为反应及信号检测载体,集合流式细胞技术、激光技术、数字信号处理技术及传统化学技术为一体的新型生物分子检测技术。它充分利用发展成熟的流式细胞术检测原理,对目标分子(如核酸、蛋白质等)进行高通量分析。目前已在生物医学研究中得到了广泛的应用。现就其技术原理、特点及其在泪液细胞因子检测中的应用作简要介绍。
Liquichip（Multiplexed microsphere-based flow cytometric assays） is a new biomolecular detection technology using encoded microspheres suspended in liquid as reaction and detection carrier,which is an integration of flow cytometry,laser technology,digital signal processing and traditional chemical technology.It is a high-throughput analyzer for the target molecular（e.g.nucleic acid and protein） based on the principles of flow cytometry.Liquichip is now widely used in biomedical analyses.This article briefly reviews the principle and characteristic of liquichip and its application in tear cytokine analysis.
基金
广州市科技计划项目(2007J1-C0101)
