摘要
目的研究孕期维生素D(VitD)缺乏对子代大鼠肺形态发育及血小板源性生长因子-A(PDGF-A)表达的影响。方法雌性Sprague-Dawley(SD)大鼠随机分为对照组、VitD缺乏模型组(每组6只)。对照组正常饲养;模型组予以避光、不含VitD的饲料喂养,2周后与成熟SD雄性大鼠交配,每组取孕20 d的胎肺及生后1 d新生鼠肺,光镜及电镜下观察肺形态结构,RT-PCR及Western blot分别检测肺组织PDGF-A mRNA及蛋白水平的表达。结果光镜下,模型组子鼠肺泡平均表面积、平均呼吸膜周径均小于对照组(P<0.05),平均肺泡间隔厚度大于对照组(P<0.05);电镜下,模型组子鼠的板层小体数量明显少于对照组,成熟细胞器较少见。RT-PCR及Western blot结果显示模型组子鼠肺组织PDGF-A mRNA和蛋白表达水平均低于对照组(P<0.05)。结论孕期VitD缺乏抑制孕晚期胎鼠及新生大鼠的肺形态发育。VitD缺乏能显著抑制肺组织PDGF-A表达;PDGF-A表达减低可能是VitD缺乏抑制大鼠肺发育的重要机制之一。
Objective To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A（PDGF-A） expression in rat offspring.Methods Sprague-Dawley（SD） female rats were randomly divided into two groups： normal control and vitamin D deficiency,with 6 rats in each group.The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D.After 2 weeks,the rats were mated with normal SD male rats.The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope.The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction（RT-PCR） technique and Western blot method respectively.Results Under the light microscope,smaller alveolar space,smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compared with the controls（P0.05）.Under the electronic microscope,fewer lamellar bodies but more glycogen deposition in intracytoplasm were observed in the lungs of fetal rats from the vitamin D deficiency group compared with the controls.There was an increased number of empty lamellar bodies in neonatal rats from the vitamin D deficiency group.The levels of PDGF-A mRNA and protein in lungs of fetal and neonatal rats from the vitamin D deficiency group were significantly lower than the controls（P0.05）.Conclusions Maternal vitamin D deficiency during pregnancy may inhibit the development of lung morphogenesis and PDGF-A expression in late fetal and neonatal rats.The low expression of PDGF-A may be involved in the inhibitory effect of vitamin D deficiency on the lung development.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2011年第4期326-330,共5页
Chinese Journal of Contemporary Pediatrics
基金
南京市卫生局医学科技发展项目(ZKM06045)
南京医科大学重点发展项目(06NMU2024)
