摘要
为了探究表面活性剂与氧载体对地衣芽孢杆菌ZJUEL31410发酵产弹性蛋白酶的影响,将各种不同浓度的油酸、液体石蜡、乙酸乙酯、吐温80、正十二烷添加至发酵培养基,对发酵过程中弹性蛋白酶活、生物量、还原糖消耗量等指标进行考察,结果发现添加4%吐温80时酶活力最高可达515U/mL,而其它表面活性剂的效果不如吐温80。本试验中还利用5L发酵罐对吐温80添加时间进行研究,结果表明发酵初始添加吐温80对弹性蛋白酶合成有较好的效果。从细菌微观形态的变化分析得出结论：吐温80可以增强细胞膜的通透性,使溶氧更易进入细胞,从而促进弹性蛋白酶的合成。
In order to research the effect of surfactants and oxygen vectors on elastase produced by Bacillus licheniformis ZJUEL31410,different concentration of oleic acid,liquid paraffin,ethyl acetate,Tween 80 and dodecane on elastase production,biomass,reducing sugar consumption were investigated in this study.It was found that when concentration of Tween 80 is 4%,the elastase activity reached 515 U/mL,while other additives showed less positive effect.The addition time of Tween 80 was also studied in this work using 5 L fermentor.Results showed that Tween 80 added at the beginning of fermentation had a better effect on elastase production.Based on the analysis of morphological changes,Tween 80 can enhance cell membrane permeability,which made more dissolved oxygen into the cells,thereby promoting the synthesis of elastase.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第2期17-23,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省自然科学基金项目（Y307405）
