摘要
[目的]研究肺结核病人酶联免疫斑点技术(ELISPOT)和结核菌素纯蛋白衍生物(PPD)皮试结果的相关性。[方法]24例痰结核菌培养阳性肺结核病人,38例临床疑似肺结核病人,取外周血分离获取单个核细胞(PBMCs)后行ELISPOT试验。[结果]痰结核菌培养阳性肺结核组与临床疑似肺结核组PPD皮试阳性率相似;痰结核菌培养阳性肺结核组的ELISPOT阳性率高于临床疑似肺结核组;ELISPOT斑点数与PPD皮试反应程度之间缺乏相关性。[结论]ELIS-POT的诊断特异性高于PPD皮试。
Objective：To study the correlation between ELISPOT and PPD skin test results in patients with pulmonary tuberculosis.Methods：After isolated PBMCs from blood,the TB-specific ELISPOT skin test were performed in 24 patients with bacteria-confirmed pulmonary tuberculosis and 38 patients with clinically suspected pulmonary tuberculosis.The results were analyzed statistically.Results：Comparative analysis showed that 24 confirmed TB patients and 38 suspected TB patients had similar positive rate when PPD skin test was performed,but significantly different in positive rate when ELISPOT skin test was used,ELISPOT was higher than PPD.The number of spots in ELISPOT assay and the degree of reaction in PPD skin tests were short of correlation in this group of TB patients.Conclusion：The specificity of TELISPOT is higher than PPD skin test.
出处
《护理研究（中旬版）》
2011年第4期953-955,共3页
Chinese Nursing Researsh
关键词
肺结核
PPD皮试
酶联免疫斑点实验
相关性
pulmonary tuberculosis
PPD skin test
enzyme-linked immunospot assay
correlation
