摘要
为探讨炔雌醚对雄性长爪沙鼠生殖器官及繁殖的影响,将试鼠随机分为多剂量组、单剂量组和对照组。多剂量组以每次3.5mg/kg·BW(5次/周,2周)、单剂量组以35mg/kg·BW炔雌醚灌胃。处理后15d、30d、60d、90d剖检,观察性腺系数、精子质量和繁殖率等指标,H·E染色观察附睾组织病理学变化。结果表明:与对照相比,炔雌醚处理后15d、30d给药组试鼠睾丸、附睾及精囊腺极度萎缩(P<0.01),精子密度、精子活力和活精子百分率明显下降(P<0.01),精子畸形率显著上升(P<0.01),附睾管腔内充满大量发育异常的精子细胞,繁殖率显著下降;与单次给药相比,多次连续给药对试鼠生殖器官影响更严重。处理后60d,除多剂量组附睾外,各组性腺基本恢复至对照水平,单剂量组、多剂量组精子质量及繁殖率与对照仍有极显著差异(P<0.01)。90d后试鼠各项指标及繁殖率均恢复正常,表现为可逆性。结果表明:该药对长爪沙鼠不育效果明显;多次低剂量给药较单次高剂量给药不育效果更好;90d后,炔雌醚对生殖器官和繁殖的影响能够恢复正常。
In this study,Mongolian gerbils were randomly divided into multi-dose treated group（MDT group）,one-dose treated group（ODT group） and control group（CK group） to explore effects of quinestrol on reproductive organs and reproduction.The epididymides,seminal vesicles and semen quality,fertility were assessed at 15,30,60 and 90 days after treatment.Histopathological changes of epididymides were observed by H.E stain.Results showed that the coefficient of testes,epididymides,and seminal vesicles were significantly decreased（P0.01） at 15 or 30 days after treatment.The normal function of the sex gland was impaired,which led to significant decrease in the density and amount of live spermatozoa and reproductive rates（P0.01）,as well as a significant increase of abnormal spermatozoa（P0.01） in both MDT group and ODT group.Compared with the ODT group,the damage for gerbil reproduction in MDT group was more severely at 15 or 30 days after treatment（P0.01）.After 60 days of treatment withdrawal,the sex gland in treated group was normalized except for gerbil epididymides in the MDT group,while semen quality and reproductive rates were still lower than those in the control group（P0.01）.The physiological indicators were recovered by 90 days of treatment withdrawal.This indicates that quinestrol shows certain effects on reducing the fertility of male gerbil with a reversible effect after 90 days＇ treatment and multi-dose treatment has more effects to infertility compared with one-dose treatment.
出处
《兽类学报》
CAS
CSCD
北大核心
2011年第2期171-178,共8页
Acta Theriologica Sinica
基金
"973"鼠类种群生殖调控与不育控制机理资助项目(2007CB109105)
关键词
炔雌醚
长爪沙鼠
不育
可逆性
Infertility
Mongolian gerbil（Merione sunguiculatus）
Quinestrol
Reversibility
