摘要
目的了解福建省流行的麻疹野病毒基因型别及特征。方法应用B95a细胞[Epstein-Barr(EB)Virus-Transformed,Marmoset B Lymphoblastoid Cell Line,埃泼斯坦-巴尔病毒转化的绒猴淋巴母细胞]或Vero/SLAM细胞[Vero Cells Transfected to Express the Human Signaling Lymphocyte Activation Molecule(SLAM),淋巴信号激活因子转染的非洲绿猴肾细胞],从疑似麻疹病例的咽拭子或尿液标本分离麻疹病毒,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)扩增分离株病毒的核蛋白(Nucleoprotein,N)基因3'端450个核苷酸片段,分析其基因型别及遗传特征。结果 2002年、2006～2009年,共从6个设区的市分离了24株麻疹野病毒,均为H1基因型、H1a基因亚型,未分离到其他型别的毒株。福建株间核苷酸同源性为97.8%～100.0%,在遗传树图中属于4个独立分支谱系(Lineage),Lineage4分支病毒出现了3个新的氨基酸突变位点。福建株中来自不同地区和年份的MV(Measles Virus,麻疹病毒)07-29FZ(Fuzhou,福州)、MV07-20ND(Ningde,宁德)、MV07-47ND和MV08-78QZ(Quanzhou,泉州)、MV06-32LY(Longyan,龙岩)、MV07-30FZ、MV07-39FZ和MV07-46ND的N基因3'端的450核苷酸同源性均为100.0%。福建株与H1基因型中国代表株、H2基因型及疫苗株S(Shanghai,上海)191相比,与S191疫苗株的A基因组差异最大(核苷酸同源性90.1%～93.3%,氨基酸同源性86.8%～91.4%)。结论 H1a为福建省本土麻疹野病毒优势基因亚型,未发现其他基因亚型。近3年的分离株出现了新的氨基酸位点突变。不同地区或同一地区内存在多传播链同时循环,也存在同一野病毒株在不同地区、不同年份持续循环。
Objective To analyse of the characteristic of the indigenous measles virus （MV）in Fujian before 2012. Methods MV were isolated from the throat swabs or urine specimens of measles patients by the B95a or Vero/SLAM cell line and identified by RT-PCR. The 450 nucleotide sequence of C terminus of the nucleoprotein （N） gene was amplified and directly sequenced. Phylogenetic analysis were performed. Results 24 strains were isolated from Fuzhou, Ningde, Quanzhou, Longyan and Sangming cities during 2002, 2006-2009 . All the isolates were all identified as H1 genotype, H1a subgenotype, which belong to 4 different Lineages in the phylogenetic tree. Comparing with the other strains in the H1a, the strains of Lineage 4 got 3 new mutation sites of Amino Acids. The sequence identity matrix of the Fujian isolates showed that the homology of nucleotide sequence was 97.8% -100%. Fujian strains of MV07-29FZ （ Fuzhou, FZ）, MV07-20ND （ Ningde, ND ）, MV07-47ND and MV08-78QZ （ Quangzhou, Qz）, MV06-32LY （Longyan, LY）, MV07-30FZ, MV07-39FZ and MV07-46ND, which isolated from different years and prefectures, had the same 3＇ terminal sequence of 450 nucleotide respectively. The homology of nucleotide and amino acid sequences were 90.1%-93.3% and 86.8%-91.4% respectively compared with genotype A （including S191）. Conclusions H1a sub-genotype was the pre-dominant genotype of wild measles virus circulated in Fujian Province during 2002, 2006-2009, other sub-genotypes were not found. Strains isolated in recent 3 years got new mutation sites of amino acids. There were several different transmission chains caused measles co-circulations in the same or different prefectures in Fujian province, and there was one wild MV which could induce outbreaks or epidemiology in different cities for several years.
出处
《中国疫苗和免疫》
CAS
2011年第2期147-151,共5页
Chinese Journal of Vaccines and Immunization
关键词
麻疹病毒
遗传分析
Measles virus
Phylogenetic analysis
