摘要
从石油污染的土壤中筛选出一株能产生细菌素类物质的芽孢杆菌,命名为SLY—3。该菌株分泌的活性物质抑菌活性好,对细菌主要是革兰氏阳性菌、霉菌都有抑制作用。从表型、生理生化反应及16S rDNA序列比对方面进行分析,最终确定菌株SLY—3为枯草芽孢杆菌(Bacillus subtilis)。培养基初始pH为7.0,28℃振荡培养24 h后,发酵产物抑菌活性最高。
One Bacillus strain named SLY—3 producing bacteriocin-like substance（BLS） was screened from the soil polluted by oil.The G＋ bacteria and molds were effectively inhibited by BLS produced by strain SLY—3.With the analysis of colony morphology,physiological and biochemistry experiments and 16S rDNA gene sequence,the strain SLY—3 was identified as Bacillus subtilis.The BLS secreted by Bacillus subtilis SLY—3 has the highest inhibitory activity when it is cultured for 24 h at initial pH 7.0 and 28 ℃.
出处
《科学技术与工程》
2011年第12期2644-2648,共5页
Science Technology and Engineering
基金
中国烟草总公司山东省公司科技计划项目(KN172)资助
关键词
细菌素
枯草芽孢杆菌
鉴定
抑菌活性
bacteriocin Bacillus subtilis identification anti-microbial activity
