摘要
目的:优选薄荷中总黄酮的提取工艺。方法:以薄荷为原料,以芦丁为标准品,以乙醇为溶剂,通过正交实验对薄荷黄酮的提取进行研究,并用分光光度法测定黄酮类物质的含量。结果:影响薄荷总黄酮提取效果的主次因素为:乙醇浓度>提取时间>固液比>提取温度。结论:黄酮最佳提取工艺为乙醇浓度95%,固液比1:15,提取时间1h,提取温度80℃。
Objective：To study the optimum methanol extraction process of total flavonoids in Mentha haplocalyx.Methods：With Rutin as a standard and the Mentha haplocalyx as the subject,the paper studied the extraction of the flavonoids from Mentha haplocalyx with orthogonal test.It was extracted by ethanol solvent.And the percent was tested by spectrophotometer.Results：The factors which influenced the extraction rate were sorted as follows：concentration of ethanol reaction time ratio of liquid to solid reaction temperature.Conclusion：The optimum extracting conditions were：ethanol concentration 95%,the ratio of liquid to solid as 15∶1,reaction time 1h,reaction temperature 80℃.
出处
《辽宁中医药大学学报》
CAS
2011年第5期64-66,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
广东省科学事业费计划项目(2008B071101032)
关键词
薄荷
黄酮
乙醇提取
正交实验
分光光度法
Mentha haplocalyx
flavonoid
alcohol extraction
orthogonal test
spectrophotometry
