摘要
目的探讨凋亡抑制基因Survivin和抑癌基因PTEN在原发性肝细胞癌及癌旁组织中的表达及其相关性。方法应用RT-PCR法,对50例原发性肝细胞癌和34例癌旁组织中Survivin和PTEN基因的表达情况进行定量检测。结果 Survivin在原发性肝细胞癌组织中高表达,而在癌旁组织中无表达;原发性肝细胞癌中PTEN的表达量明显低于癌旁组织;Survivin和PTEN的表达量与原发性肝细胞癌的分化程度有关,与患者发病年龄、肿瘤大小、门静脉癌栓、肝内转移无关;随着恶性程度加重,Survivin和PTEN基因表达呈显著负相关。结论 Survivin和PTEN在原发性肝细胞癌发生、发展中起着不同的作用,联合检测对原发性肝细胞癌的早期诊断、判定预后提供一定的临床依据。
Objective：To investigate the expression of Survivin and PTEN gene and their relationship in primary hepatocellular carcinoma and adjacent tissues.Methods：The expression of Survivin and PTEN were determined in 50 cases of hepatocellular carcinoma and 34 cases of corresponding noncancerous tissues by RT-PCR,and the quantitative detection were followed.Results：Survivin in primary hepatocellular carcinoma is highly expressed in cancer tissues and no expression in corresponding noncancerous tissues;the expression of PTEN in primary hepatocellular carcinoma was significantly lower than the adjacent tissues;The expression of Survivin and PTEN were significantly correlated with histological differentiation,but not with patients age,tumor size,portal vein tumor thrombus or intrahepatic metastasis;With the degree of malignancy increased,The expression of Survivin and PTEN gene was significantly negatively correlated.Conclusion：Survivin and PTEN may play different roles in the oncogenesis and progression of primary hepatocellular.Detection of Survivin together with PTEN can supply certain clinical evidence for early diagnosis of hepatocellular carcinoma and prognosis.
出处
《中国优生与遗传杂志》
2011年第5期17-19,共3页
Chinese Journal of Birth Health & Heredity
基金
黑龙江省教育厅科学技术研究项目
No11541371
