摘要
背景:移植肝急性排斥反应是导致移植物功能损伤的主要危险因素,目前诊断主要靠肝组织穿刺活检,带来感染、出血等风险。如何无创、简单、准确地进行诊断成为当前该领域的热点。目的:观察分析白细胞介素2、白细胞介素10、人类白细胞抗原G的表达与人类肝移植急性排斥之间的关系。方法:用酶联免疫方法检测59例肝脏移植受者外周血白细胞介素2、白细胞介素10、人类白细胞抗原G的表达情况。将受者按照有无急性排斥反应分为急性排斥组和非排斥组;并将非排斥组按肝功能情况分为肝功能正常组与异常组。结果进行统计学分析,并绘制ROC曲线,比较曲线下面积及其临界值的敏感性和特异性。结果与结论:急性排斥组外周血中白细胞介素10、人类白细胞抗原G表达均低于与非排斥组(P=0.032,0.002),而排斥组中白细胞介素2高于非排斥组(P=0.002)。肝功正常组与异常组间白细胞介素10、人类白细胞抗原G表达差异无显著性意义(P=0.525,0.084),而肝功能异常组白细胞介素2表达高于肝功能正常组(P=0.02)。ROC曲线下面积为人类白细胞抗原G>白细胞介素2>白细胞介素10。结果提示,白细胞介素2、白细胞介素10、人类白细胞抗原G的表达都与排斥相关,三者中人类白细胞抗原G为诊断急性排斥的最佳指标。
BACKGROUND：Liver acute rejection is one of the most severe complications that may blast the graft.Now liver biopsy is the main way to diagnose it,which may bring about some complications such as infections and hemorrhages.So it is one of the most important focal points in the field of liver transplantation to discover a non-invasion or less-invasion index to diagnosis and forecast of liver acute rejection.OBJECTIVE：To explore the relationship of the expression of interleukin-2,interleukin-10,human leukocyte antigen-G（HLA-G） and acute rejection in liver transplantation.METHODS：Fifty-nine blood samples were taken from cases after liver transplantation.The expression of interleukin-2,interleukin-10 and HLA-G was assayed by ELISA.The cases were divided into the acute rejection group and non-rejection group,and the latter group was divided into normal liver function group and abnormal group.The results were analyzed and ROC curve was made.Area under ROC curve and the sensibility and specificity of the cut-off point were analyzed statistically.RESULT AND CONCLUSION：The expressions of interleukin-10 and HLA-G in serum of the acute rejection group was obviously lower than those in the non-rejection group（P=0.032,0.002）,while expression of interleukin-2 in acute rejection group was higher than that in the non-rejection group（P=0.002）.The expression of interleukin-2 was statistically higher in the abnormal liver function group than that in the normal liver function group（P=0.02）,while the expression of interleukin-10 and HLA-G had no statistical difference in the two groups（P=0.525,0.084）.The expression of interleukin-2,interleukin-10 and HLA-G is connected with liver acute rejection.And in the three factors HLA-G is the most effective index for diagnosis and forecast of liver acute rejection.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第53期9917-9920,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
