摘要
目的:筛选贞清胶囊的水提醇沉最佳工艺条件。方法:高效液相色谱法测定水煎液和醇沉液中马钱苷的含量;以马钱苷提取率和干膏率为评价指标,采用正交设计法考察加水量、水提时间、水提次数等因素对水提工艺的影响;考察醇沉前药液相对密度、醇沉浓度、醇沉时间三因素对醇沉工艺的影响。结果:最佳水提工艺条件为加入8倍量水,提取2次,每次1.5h(100℃);最佳醇沉工艺条件即加入乙醇使含醇量达60%,水提液浓缩体积比为1∶1.2,醇沉时间24h。结论:经正交设计筛选贞清胶囊的水提醇沉工艺是科学可行的。
OBJECTIVE To optimize the process of extraction and purification of Zhenqing capsules by orthogonal design. METHODS The contents of loganin of Zhenqing capsules in water solution were detected by HPLC. The content of loganin and rate of dry were determined and taken as the assessment index; the effects of three factors, water volume, extraction time, extraction times on extraction were evaluated by orthogonal design. The effects of another three factors, alcohol volume, concentra tion of condensed solution, reaction time on content of Zhenqing capsules of alcohol precipitation were also evaluated by orthogonal design. RESULTS The optimum water extraction process was as follows： water was used as the extracting solvent, two times of extracting 1.5 h each time,8 times solvent to the decoction pieces （100℃）; the best alcohol precipitation process was that 60% of precipitation concentration,concentrated volume ratio of extraction is 1：1.2,alcohol deposit lasted 24 hours. CONCLUSION The water-extraction and alcohol-precipitation process of Zhenqing capsules by orthogonal design is scientific and feasible.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第8期619-621,共3页
Chinese Journal of Hospital Pharmacy
基金
国家自然基金资助项目(编号:30672730)
关键词
正交设计
水提醇沉工艺
马钱苷
orthogonal design
the wate-extraction and alcohol precipitation process
loganin
