摘要
利用碱法和盐法提取薏米蛋白质,对薏米蛋白提取方法进行优选。以蛋白提取率为指标,碱法以提取液pH值、料液比、提取温度及提取时间进行单因素试验,盐法以盐质量分数、料液比、提取温度及提取时间进行单因素试验;通过L9(34)正交试验优化提取工艺条件,并对提取方法进行比较。结果表明:碱法优于盐法,碱法最佳提取条件为料液比1:12(g/mL)、提取温度35℃、提取时间5h、pH11,在此条件下,蛋白质的提取率可达43.56%。
Two extraction methods,namely alkaline method and salt method were used to extract protein from Job s Tears seeds, and their effects on the extraction rate of protein from Job s Tears seed were compared.In the alkaline extraction method,the extraction parameters pH,material-to-liquid ratio,temperature and extraction time were optimized by single factor and orthogonal array design methods based on the extraction rate of protein from Job s Tears seed.The extraction parameters optimized（also by single factor and orthogonal array design methods）in the salt extraction method were salt concentration,material-to-liquid ratio,temperature and extraction time.The results showed that the optimum material-to-liquid ratio,temperature,extraction time and pH for the alkaline extraction of Job s Tears seed protein were 1：12,35℃,5 h and 11,respectively.Under these conditions,the extraction rate of protein was up to 43.56%.Overall,the alkaline extraction method was superior to the salt extraction method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第8期88-92,共5页
Food Science
基金
辽宁省教育厅高等学校科学技术研究项目(2009A811L20100154)
辽宁省科技厅农业攻关计划项目(2009209001)
辽宁大学"211工程"三期建设项目
关键词
薏米
蛋白质
提取
Job s Tears seed
protein
extraction
