摘要
研究培养时间对菌丝体内壳聚糖及其它主要成分含量的影响及产物的收率。方法：培养 ４８ ｈ，菌丝体数量很快增长，菌丝体干重达到 １３ ｇ／Ｌ培养基。甲壳素和壳聚糖的分离包括：用 ２％氢氧化钠溶液在 ９０℃条件下除蛋白质２ｈ，再用１０％乙酸６０℃、２ｈ提取壳聚糖，然后在ｐＨ９．０条件下沉淀。结果：鲁氏毛霉经２ｄ培养，甲壳素和壳聚糖产率分别为菌丝体干重的８．８％和７．４％。结论：鲁氏毛霉菌丝体可作为用于医药、化妆品等的甲壳素和壳聚糖的来源。
Purpose: The objectives of the present study were to examine the influence of growth time on the contents of chitosan and other main components of M. rouxii mycelia, as well as the yield of chitin and chitosan during the isolation process. Methods: The mycelia yield increased rapidly up to 48 h of growth. The final density of the culture during this time was about 13 g of dry mycelia per litre of medium. Chitin and chitosan isolation involved deproteinization of the mycelia with 2% NaOH solution at 90℃ for 2 h, extraction of chitosan with 10% acetic acid at 60℃ for 6 h and subsequent precipitation of chitosan at pH 9. 0. Results: The mycelia of M. rouxii from 2 day old cultures may serve as a source of chitin and chitosan obtained with yields 8. 8% and 7. 4%, on a dry weight basis of mycelia, respectively. Conclusion: Mycelia of M. rouxii may be used as a source of chitin and chitosan for medical, cosmetic and other purpose.
出处
《中国生化药物杂志》
CAS
CSCD
1999年第5期227-229,共3页
Chinese Journal of Biochemical Pharmaceutics
