摘要
试验合成了一种荧光试剂对-氨-苯基卟啉(TAPP),该试剂与钴(Ⅱ)离子和镍(Ⅱ)离子反应发生荧光淬灭,据此试验了以其为探针测定痕量钴(Ⅱ)和镍(Ⅱ)的荧光分光光度法。在pH7.2的Tris-盐酸缓冲介质中,在一定量的十二烷基硫酸钠(SDS)存在下,通过测定样品溶液和空白溶液在激发波长392 nm和发射波长516 nm处的发射荧光强度F_s及F_0,计算得反应液荧光强度减弱程度△F,其结果表明:△F与钴(Ⅱ)和镍(Ⅱ)的浓度均在2.0×10^(-7)～1.0×10^(-5)mol·L^(-1)范围内呈线性关系,方法的检出限(3S/N)分别为2.0×10^(-8)mol·L^(-1)和4.0×10^(-8)mol·L^(-1)。此方法用于自然水体和污水中钴(Ⅱ)和镍(Ⅱ)的测定,回收率分别在97.0%～108.5%和97.0%～106.5%之间。
A fluorescence reagent, 5, 10, 15,20-tetra（4-aminophenyl）-porphyrin （TAPP） was synthesize＆ Fluorescence quenching effect was observed when TAPP was reacted with Co（ Ⅱ） or Ni（ ]Ⅱ）. The use of TAPP as a fluorescence probe in the determination of trace amounts of Co and Ni was studied. In a Tris-HCl buffer medium of pH7. 2, Co（ Ⅱ ） and Ni（ Ⅱ） were reacted with TAPP in the presence of SDS, the magnitude of decrease in fluorescence intensity, AF, of the reaction was obtained by taking the difference between values of emission fluorescence intensity of the blank solution （F0） and the sample solution （Fs） measured at （λem） 516 nm, with （λes） at 392 ran. Linear relationships between values of AF and concentration of Co（ Ⅱ） and Ni（Ⅱ ） were found to be same in the range of 2. 0×10^-7～1.0×10^-5mol·L^-1, with their detection limits （3S/N）of 2. 0×10^-8mol·L^-1 and 4.0×10^-8mol·L^-1 respectively. Values of recovery found in the analysis of samples of natural water and waste water, were in the range of 97. 0 %-108. 5 % for Co（ Ⅱ） and 97. 0%-106. 5% for Ni（ Ⅱ）.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2011年第3期302-304,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家"863"项目(2006AA06Z407)
湖南省自然科学基金(06JJ20062)
