摘要
目的 了解苯接触对热应激蛋白（ＨＳＰｓ） 表达的影响以及ＨＳＰｓ 在苯毒性机理中的作用， 探讨ＨＳＰｓ 作为接触苯工人分子生物标志物的可能性。方法 选择接触苯浓度≥４０ｍｇ／ ｍ３ 工人４２ 名为高暴露组， 接触苯浓度＜ ４０ｍｇ／ｍ ３工人５０ 名为低暴露组， 不接触苯等职业有害因素工人４２ 名为对照组。采用Ｗｅｓｔｅｒｎｄｏｔｂｌｏｔ 方法测定其血浆ＨＳＰ２７ 、ＨＳＰ６０ 、ＨＳＰ７０ 及ＨＳＰ９０ 水平。结果 发现高暴露组血浆ＨＳＰ７０ 水平明显高于对照组（ Ｐ＜ ０．０５） ， 而ＨＳＰ２７ 、ＨＳＰ６０ 和ＨＳＰ９０ 在四组间差异无显著意义。结论 血浆ＨＳＰ７０ 水平能反映工人接触苯的水平及反应能力， 作为分子生物标志物在苯作业工人健康监护中有一定价值。
Objective To investigate the effects of benzene exposure on the expression of heat shock proteins(HSPs) and the probability of HSPs in plasma of workers as biomarkers.Method 144 workers were divided into three groups according to the concentration of benzene in working environment:the high exposed group had 42 workers who exposed to a high benzene exposure(≥40mg/m 3),the low exposed group had of 50 workers exposed to a low level benzene exposure(<40mg/m 3),and the control group consisted of 42 workers who neither had benzene nor other known harmful factors exposure.Using Western dot bloting,the levels of HSP27,HSP60,HSP70 and HSP90 in plasma were determined.Results The levels of plasma HSP70 in high exposed groups were significantly higher than that in controls(P<0 05).Conclusion The level of plasma HSP70 might be used as an effective biomarker in the health monitoring of benzene workers.
出处
《中国工业医学杂志》
CAS
1999年第5期260-262,共3页
Chinese Journal of Industrial Medicine
基金
国家自然科学基金
卫生部科研基金
