摘要
以蚕豆为实验材料,利用药理学实验和分光光度法,研究了ABA处理及ABA与H2S合成抑制剂共处理对蚕豆气孔运动的影响,以及体内H2S水平、H2S合成酶L-/D-半胱氨酸脱巯基酶(磷酸吡哆盐依赖性酶)活性变化.结果表明:(1)光下H2S的合成抑制剂羧甲氧基胺半盐酸盐(AOA)、羟氨(NH2OH)、L-/D-半胱氨酸脱巯基酶分解产物C3H3KO3+NH3均明显抑制ABA诱导的蚕豆气孔关闭;(2)外源ABA能够明显提高叶片的H2S水平及L-/D-半胱氨酸脱巯基酶活性;(3)AOA、NH2OH、C3H3KO3和NH3均可以逆转ABA所引起的H2S水平及L-/D-半胱氨酸脱巯基酶活性的升高.研究发现,ABA可通过增强L-/D-半胱氨酸脱巯基酶活性,促进L-/D-半胱氨酸分解生成H2S,进而诱导蚕豆气孔关闭.
Through pharmacological combined with spectrophotography to study the effects of ABA and ABA with inhibitors of H2S synthesis on stomatal closure of Vicia faba L.,H2S generation and L-/D-cysteine desulfhydrase（pyridoxalphosphate dependent enzymes） activity.The results showed that AOA and NH2OH,inhibitors of pyridoxal-5′-phosphate-dependent enzymes,C3H3KO3＋NH3,products of L-/D-cysteine desulfhydrase all inhibited ABA-induced stomatal closure of Vicia faba L.obviously under light condition.Treatment with ABA induced H2S generation and increased L-/D-cysteine desulfhydrase activity.AOA,NH2OH,C3H3KO3＋NH3 reduced H2S generation induced by ABA and the activity increase of L-/D-cysteine desulfhydrase.Those results suggested that ABA enhanced the activity of L-/D-cysteine desulfhydrase,inducing the accumulation of H2S via L-/D-cysteine resolving,and finally induced stomatal closure of Vicia faba L..
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第2期298-304,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30970288)
山东省自然科学基金(ZR2010CM024)
植物生理学与生物化学国家重点实验室开放课题(SKLPPBKF09001)
关键词
蚕豆
硫化氢
脱落酸
气孔运动
Vicia faba L.
hydrogen sulfide
abscisic acid
stomatal movement
