摘要
目的探讨激活素A(Activin A)及激活素结合蛋白(Follistatin,FS)在急性酒精性肝损伤小鼠肝组织中的表达。方法通过1次/12 h给予小鼠5 g/Kg酒精连续灌胃3次,复制急性酒精性肝损伤小鼠模型,采用实时定量RT-PCR检测小鼠肝组织Activin A及FS mRNA表达水平,免疫组织化学染色观察小鼠肝组织Activin A及FS蛋白的表达水平。结果急性酒精性肝损伤小鼠肝组织Activin A mRNA和蛋白表达水平均显著高于对照组(P<0.01);FS mRNA和蛋白表达水平与对照组比较,差异均无统计学意义(P>0.05)。结论急性酒精性肝损伤小鼠肝组织Activin A-FS表达失衡,以Activin A升高为主,提示Activin A-FS系统失衡可能与酒精性肝损伤有关。
Objective To investigate the expression of activin A and activin-binding protein（Follistatin,FS） in liver tissues of mice with acute alcohol-induced liver lesion.Methods Mice were administered with alcohol by gavage at a dosage of 5 g / kg,once 12 h for 3 times,to copy the mouse model of acute alcohol-induced liver lesion.The transcription levels of activin A and FS mRNAs in liver tissues of mice were determined by real-time quantitative RT-PCR,while the expression levels of activin A and FS by immunohistochemical staining.Results Compared with those in control group,the mRNA and protein levels of activin A in liver tissues of model mice were significantly high（P 0.01）,while those of FS showed significant difference（P 0.05）.Conclusion The expression of activin A-FS in liver tissues of mice with acute alcohol-induced liver lesion was unbalanced,which was mainly expressed as increased activin A,indicating that the unbalance of activin A-FS system might be associated with alcohol-induced liver lesion.
出处
《中国生物制品学杂志》
CAS
CSCD
2011年第3期285-287,291,共4页
Chinese Journal of Biologicals
基金
国家自然科学基金(30771957)
吉林省科技厅(20060928-01)
吉林省卫生厅(2006z010)
关键词
激活素类
激活素结合蛋白
肝疾病
酒精性
Activin
Activin-binding protein
Liver lesion
alcohol-induced
