摘要
目的研究重组人促红细胞生成素(RhEPo) 对大鼠实验性脑出血后凋亡细胞及缺氧诱导因子( hypoxia inducible factor-1α,HIF-1α) 的影响。方法随机分为脑出血组、脑出血 RhEPO 干预组、假手术组,动物模型采用自体血脑内注射的方法,其中脑出血 RhEPO 干预组为大鼠造模后给予 5 000 U/kgRhEPO腹腔注射,取组织后应用免疫组化方法检测脑出血后不同时间血肿周边脑组织中 HIF-1α蛋白表达情况,应用 TUNEL 法检测凋亡细胞。结果出血后,HIF-1α蛋白阳性表达率随时间进行性增加,并于出血后 72 h 达到高峰,此后 HIF-1α蛋白表达的阳性率逐渐下降,脑出血后不同时间点阳性率比较差异有统计学意义(P <0. 05) ; 脑出血 RhEPO 干预组较脑出血组及假手术组各时间点细胞凋亡数低及 HIF-1α蛋白阳性率低(P <0. 05) 。结论 EPO 能显著降低 HIF-1α表达,并对脑出血后脑组织有良好的保护作用。
Objective To investigate the effect of RhEPO on hypoxia inducible factor-1α（HIF-1α） and cell apoptosis in rats with experimental intracerebral hemorrhage（ICH）.Methods 152 rats were randomly divided into cerebral hemorrhage group,RhEPO intervention group and sham operation group,and the animal models were established by autoblood intracerebral injection.The rats in RhEPO intervention group were given 5 000 U/kg RhEPO by intracerebral injection after ICH models were sut up.The expressions of HIF-1α in periphery tissue of cerebral haematoma at different time points after ICH were detected by immunohistochemical method（SP） and the cell apoptosis was detected by TUNEL.Results The expression rate of HIF-1α after ICH was increased with time,which reached peak on 72h after ICH,then it was decreased gradually.There was a significant difference in the expression rate among different time points（P0.05）.The apoptosis cell number and expression positive rate of HIF-1α at different time points in RhEPO intervention group were significantly lower than those in cerebral hemorrhage group and sham operation group（P0.05）.Conclusion RhEPO can significantly reduce the expression rate of the HIF-1α and cell apoptosis after ICH,which can protect the brain tissue after ICH.
出处
《河北医药》
CAS
2011年第5期645-647,共3页
Hebei Medical Journal
关键词
促红细胞生成素
缺氧诱导因子
细胞凋亡
脑出血
RhEPO
hypoxia inducible factor-1α
cell apoptosis
intracerebral hemorrhage
