摘要
目的:建立一种接近临床发病机制的腰椎间盘退变模型,方法:选用20只清洁级成年日本大耳白兔,按体质量编号,随机分为2组,即模型组和对照组,各10只。模型组麻醉后,手术造成腰椎间不稳;并加以异常应力,以促进造模。对照组同法麻醉,作皮肤切口后直接缝合;两组动物在相同条件下饲养,于术后1个月、2个月随机各取5只兔子行MRI及病理学检查。结果:术后2个月模型组兔腰椎间盘MRI检查出现椎间盘退变的改变;而对照组兔腰椎间盘信号无明显改变。造模2个月后,对照组兔腰椎间盘病理学检查无明显退变表现;而模型组造模2个月后,出现纤维环断裂,髓核向后突出,终板软骨钙化层增厚,透明软骨变薄、软骨细胞坏死增多等退变表现。结论:脊柱动、静力平衡失调(椎间不稳),加上异常应力刺激,可稳定地诱发出兔腰椎间盘退变模型;该方法是间接性微小损伤累积发展,更贴近临床椎间盘退变的发生机制,因此该方法是良好的椎间盘退变造模方法。
Objective： To establish an animal model of degeneration of the intervertebral disc to imitate human pathogenesis.Methods： Twenty adult Japanese white rabbits were randomized into the model group and control group,10 in each.Under anesthesia,rabbits in the model group were operated to destabilize lumbar intervertebral disc and then given extra-mechanical load to promote the injury;and rabbits in the control group were sutured back just after incision.Both groups were fed in the same conditions and every 5 rabbits in each group were sacrificed to perform MRI and pathological examination in 1 and 2 months after surgery.Results： In 2 months after surgery,MRI showed degeneration in the lumbar intervertebral disc and typical pathological changes of degeneration were observed in the model group.No degeneration was observed in the control group.Conclusion： Destabilizing the lumber disc plus mechanical loading can steadily induce degeneration of the intervertebral disc in rabbits.The development of pathological changes in this model is close to the degenerative disc disease in humans.
出处
《浙江中西医结合杂志》
2011年第3期161-162,164,F0003,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省中医药科技计划项目(N0.2008CA104)
关键词
兔
腰椎间盘退变
动物模型
rabbits degeneration of the lumber intervertebral disc animal model
