摘要
目的了解慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)含量和PBMCs中初始T细胞比例,分析两者的相关关系。方法采集CHB患者和健康人外周静脉血标本,(1)应用实时荧光定量聚合酶链反应(RT-PCR)技术检测TRECs含量;(2)利用荧光标记抗体FITC-CD3、PE-CD45RA、PE-cy5-CD62L标记细胞表面分子,流式细胞仪检测PBMCs中CD3+CD45RA+CD62L+细胞比例;(3)TRECs含量与初始T细胞相关性分析。结果 (1)CHB组TRECs含量为(6.78±1.06)拷贝/103 PBMCs,低于对照组(8.16±2.07)拷贝/103 PBMCs,P<0.01。(2)CHB组初始T细胞比例为(20.73±6.75)%,低于对照组(27.82±9.66)%,P<0.05。(3)CHB组初始T细胞比例与TRECs含量呈正直线相关,相关系数r=0.688,P<0.01。对照组初始T细胞比例与TRECs含量正相关,r=0.646,P<0.01。两组r值比较差异无统计学意义,P>0.05。结论 (1)CHB患者PBMCs中TRECs含量与初始T细胞比例均低于正常人;(2)CHB患者与正常人PBMCs中TRECs含量与初始T细胞比例均正相关。
Objective To test the content of T cell receptor excision circles（TRECs） and the frequency of naive T lymphocytes in patients with chronic HBV infection and analyze their correlation.Methods Peripheral blood were collected from normal individuals and patients with chronic HBV infections.（1） TRECs were assessed by real-time PCR;（2） Lymphocytes were labeled with fluorescent-labeled antibodies FITC-CD3,PE-CD45RA and PE-cy5-CD62L and flow cytometry was used to detect the expressions of CD3,CD45RA and CD62L on the surface of T lymphocytes.Results（1） The TRECs（6.78±1.06） copies/103 in patients（n=25） with chronic HBV infections was significantly lower than that（8.16±2.07） copies/103 in healthy controls（n=25）（P0.01）.The frequency of nave T cells（20.73±6.75）% in patients（n=25） with chronic HBV infections was significantly lower than that（27.82±9.66）% in healthy controls（n=25）（P0.05）.（2） Both in patients with chronic HBV infections and in healthy controls,the frequency of nave T cells were in positive linear correlation with the TRECs,while the coefficient correlation were 0.688（P0.01） and 0.646（P0.01） respectively.The coefficient correlation in the two groups had no statistical differences,（P0.05）.Conclusion Both the nave T cell in peripheral blood and the TRECs in patients with chronic HBV infection were decreased compare to the healthy adult,and the two indices had better linear correlation.
出处
《临床肝胆病杂志》
CAS
2011年第2期157-160,共4页
Journal of Clinical Hepatology
关键词
肝炎
乙型
慢性
受体
抗原
T细胞
胸腺
T淋巴细胞
hepatitis B
chronic
receptors
antigen
T-cell
thymus gland
T-lymphocytes
