摘要
比较了5种大孔树脂对桑黄粗多糖的吸附和解吸效果,从中筛选出适合桑黄多糖分离纯化的树脂,并对其吸附和解吸条件进行了探讨.结果表明:D-101-I树脂纯化桑黄粗多糖较好,浓度为500 mg.L-1的多糖溶液在室温下吸附24 min时达到平衡,平衡吸附量为80 mg.g-1,原液上样,最佳上样量为6 BV;60%乙醇作洗脱剂,用量为2 BV时,富集洗脱多糖效果较好.
The characteristic of absorption and adsorption of five kinds of macroporous resins to crude polysaccharide of Phellinus linteus was studied.Suitable resin for purification of polysaccharide was screened from five resins,and its characteristic of absorption and adsorption was investigated.The results indicated that D-101-I resin had better absorption and adsorption property for purification of crude polysaccharide from Phellinus linteus,the polysaccharide solution which concentration was 500 mg·L-1 had achieved balance for 240 min at room temperature,the amount of balance adsorption was 80 mg·g-1,stock solution was sample,and the best sample volume was 6 BV,the polysaccharide can enrich when the eluant was 60% ethanol and its′ volume was 2 BV.
出处
《陕西科技大学学报（自然科学版）》
2011年第1期73-76,共4页
Journal of Shaanxi University of Science & Technology
基金
陕西省教育厅自然科学专项基金项目(08JK232)
关键词
大孔吸附树脂
桑黄
粗多糖
纯化
macroporous adsorption resin
Phellinus linteus
crude polysaccharide
purification
