摘要
采用生理生化、16S rDNA等方法鉴定菌株FM4B并对该菌株抑菌物质粗提液的性质进行了初步研究。生理生化实验显示,菌株FM4B属于短短芽孢杆菌,16S rDNA基因的测定与分析表明,FM4B与B.brevis(短短芽孢杆菌)的同源性达到99.5%,故推定FM4B为短短芽孢杆菌。实验表明抑菌物质粗提物对多种细菌、真菌有抑制作用,并且耐高温(90℃)、耐酸碱(pH2～11)、对蛋白酶K、胰蛋白酶、胃蛋白酶不敏感。
Classical physiological and biochemical methods and 16S rDNA analysis were used to identify bacillus strain FM4B and the crude extract produced by the strain was studied.Physiological and biochemical identification showed that FM4B related to Brevibacillus brevis.16S rDNA analysis showed that the 16S rDNA sequence of FM4B shared 99.5% homologies with sequence of Brevibacillus brevis from GenBank.So it is considered that the strain of FM4B belongs to Brevibacillus brevis.The crude extract had antibacterial activity against bacteria and fungi,which was stable after heated（90℃） and pH altered（pH2～11）,resistant to trypsin,proteinase K and pepsin.
出处
《上海交通大学学报（农业科学版）》
2011年第1期75-80,共6页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
安庆市科委农业发展基金
安徽省国际科技合作项目(08080703017)
合工大大学生创新性实验计划项目(2010052)
关键词
FM4B
鉴定
短短芽孢杆菌
抑菌物质
FM4B
identification
Brevibacillus brevis
antagonistic substance
