摘要
目的：测定辣椒油中辣椒素的含量,探求较合理的含量测定方法,建立准确简便、快速控制辣椒油中辣椒素的质量的方法,便于实验及生产的质量控制。方法：辣椒素含量测定采用HPLC法,色谱条件：色谱柱为kromsil-C18柱（4.6 mm×250 mm,5μm）;紫外检测波长280 nm;流动相：甲醇-水（80∶20）;流速1.0 min/mL。结果：辣椒素在50~300μg/mL范围内,回归方程为Y=3 554.2X＋31 083,r=0.999 6,线性关系良好。加样回收率平均值为99.12%,重复性RSD为0.40%。辣椒油中辣椒素含量是7.06%。结论：本方法准确、简便、快速,可准确控制辣椒油中辣椒素的质量,对于辣椒的良种选育、原料加工、辣椒素的提取精制。
Objective：To determine the capsaicin in chili oil contents,to seek a more reasonable detecting methods for its contents,to establish the accurate,simple,rapid controlling method for the quality of capsaicin in chili oil for the quality control in experiment and production.Methods：Capsaicin was determined by HPLC with the column： kromsil-C18 column（4.6 mm×250 mm,5 μ m）,UV detection wavelength： 280 nm,mobile phase： methanol-water（80∶20）,flowing velocity 1.0 min/mL.Results：Capsaicin was in the range of 50~300 μg/ml,the regression equation was Y=3554.2X＋31083,r=0.9996,which shows the good linear relation,and the average recovery was 99.12%,the repetitive RSD was 0.40%,the contents of Capsaicin in chili oil was 7.06%.Conclusion：The method is accurate,simple,rapid,can be used to control the quality of capsaicin in chili oil accurately.It is of great significance for the pepper breeding,processing of raw materials,the capsaicin extract refined,pepper and export products etc.
出处
《河南大学学报（医学版）》
CAS
2011年第1期27-31,共5页
Journal of Henan University:Medical Science
基金
河南省科技厅科技攻关项目(0524420044)
