摘要
目的从卵巢癌患者机体免疫状态和肿瘤生物学行为两方面来探讨患者生存影响因素。方法对获得随访的106例卵巢浆液性腺癌患者,按生存3年内和3年以上死亡分组进行统计分析。用流式细胞术(FCM)检测血清中IFN-γ、TNF-αI、L-2、IL-4I、L-5I、L-10表达水平和外周血淋巴细胞亚群CD3、CD4、CD8、CD4/CD8、CD56、CD19、CD25、CD44水平及临床病理资料。对照组检测细胞因子为51例女性职工体检健康者,检测淋巴细胞亚群及CD44表达为79例女性职工体检健康者。结果生存组IFN-γI、L-2I、L-4I、L-5高于对照组(P<0.01)I,L-10高于对照组(P<0.05)I,L-2/IL-4低于对照组(P<0.01);死亡组IFN-γI、L-2I、L-4I、L-5I、L-10高于对照组(P<0.01)I,L-2/IL-4低于对照组(P<0.01)。死亡组IFN-γ高于生存组(P<0.05)。外周血淋巴细胞亚群分析显示:生存组CD4/CD8、CD25高于对照组(P<0.01),CD56高于对照组(P<0.05),CD3、CD8低于对照组(P<0.01);死亡组CD25和CD44高于对照组(P<0.01),CD4/CD8高于对照组(P<0.05),CD3低于对照组(P<0.05)。死亡组CD44高于生存组(P<0.05)。卵巢浆液性腺癌患者的年龄,复发以及临床分期等因素与生存相关:生存组<50/≥50岁(22/23例)患者年轻多于死亡组(15/46例)(P<0.01);生存组复发/初发(15/30例)患者明显低于死亡组(35/26例)(P<0.01);生存组Ⅲ/Ⅳ期(42/3例)明显高于死亡组(46/15例)(P<0.05)。结论卵巢浆液性腺癌患者IFN-γ和CD44水平升高、患者年龄≥50岁、肿瘤复发、临床分期晚是影响患者生存的重要因素。卵巢浆液性腺癌患者机体出现Th1/Th2失衡和不同程度的免疫机制紊乱。
Purpose To evaluate the survival factors of patients with ovarian serous adenocarcinoma based on the immune status and tumor biological behaviors.Methods Total 106 patients with follow-up were divided into two groups： survival group（survived over 3 years） and death group（died within 3 years）.The levels of serum cytokines（IFN-γ,TNF-α,IL-2,IL-4,IL-5,IL-10） and lymphocyte subpopulations（CD3,CD4,CD8,CD4/CD8,CD56,CD19,and CD25）,and adhesion molecule（CD44） in peripheral blood were analyzed by flow cytometry（FCM）.Control group included 51 health women（for serum cytokine） and other 79 health women（for lymphocyte subpopulations and adhesion molecule）.Results In the survival group,the levels of IFN-γ,IL-2,IL-4 and IL-5 were higher than that in control（P0.01）,so did the level of IL-10（P0.05）,but the ratio of IL-2/IL-4 was lower（P0.01）;in the death group： the levels of IFN-γ,IL-2,IL-4,IL-5 and IL-10 were higher than that in control（P0.01）,but the ratio of IL-2/IL-4 was lower（P0.01）.The level of IFN-γ in death group was higher than that in survival group（P0.05）.In the patients,the levels of CD25 and CD56 were significantly higher than that in control（P0.01 and P0.05）,and so did the ratio of CD4/CD8（P0.01）,but the levels of CD3 and CD8 were lower（P0.01）;in the death group： the levels of CD25 and CD44 were higher than control（P0.01）,so did the ratio of CD4/CD8（P0.05）,but the level of CD3 was lower（P0.05）.Compared to the survival group,the level of CD44 in death group was higher（P0.05）.Age,recurrence and clinical stage were associated with survival： the age 50/≥50 years of patients in survival group（22/23 cases）was obviously higher than that in death group（15/46 cases）（P0.01）;and the recurrence /primary in survival group（15/30 cases） was lower than that in death group（35/26 cases）（P0.01）;The Ⅲ/Ⅳ stage in survival group（42/3 cases）was higher than that in death group（46/15 cases）（P0.05）.Conclus
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第2期142-145,149,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
卫生部科学研究基金-浙江省医药卫生重大科技计划基金(WKJ2006-2-009)
