摘要
建立黄瓜的高效再生和遗传转化体系,并进行银杏抗菌肽GK-2的遗传转化。研究结果表明,MS6(MS+0.005 mg/L TDZ+0.20 mg/L IAA)培养基适于黄瓜愈伤组织的形成和不定芽分化,7 d即可分化出不定芽,出愈率和分化率分别高达100%和86.67%。进一步用带有银杏抗菌肽GK-2目的基因的农杆菌转化黄瓜,筛选出了侵染时间20 min、共培养时间3 d以及卡那霉素抗性筛选质量浓度为100 mg/L的最佳转化体系。利用该体系转化黄瓜,获得的转化植株阳性率高达17.9%,并对黄瓜枯萎病表现出明显的抗性。
A fast regeneration system and a highly efficient genetic transformation system of cucumber were established,and the transformation of Ginkgo anti-fungal peptide GK-2 gene was also done.The result showed that MS6（MS＋0.005 mg/L TDZ＋0.20 mg/L IAA）medium was suitable for cucumber callus formation and adventitious bud differentiation,which exhibited 100% callus rate and 86.67% differentiation rate within 7 days.Transferring Ginkgo anti-fungal peptide GK-2 gene into cucumber by Agrobacterium-mediated,we obtained the optimized genetic transformation system of 20 min infection time and 3 days co-culture as well as 100 mg/L kanamycin resistance screening concentration.Using this system,the positive rate was 17.9%,and transgenic plants exhibited a high resistance to Fusarium wilt.
出处
《西北农业学报》
CAS
CSCD
北大核心
2011年第2期134-139,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(30571214)
教育部科技重点项目(20051270)
关键词
黄瓜
遗传转化
银杏抗菌肽
Cucumber
Genetic transformation
Ginkgo anti-fungal peptide
