摘要
目的研究熊果酸(Ursolic acid,UA)对人脐静脉血管内皮细胞(Human umbilical vein endothelial cells,HUVECs)增殖及ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA和蛋白表达的影响,探讨熊果酸抑制血管生长的机制。方法体外培养HU-VECs,用不同浓度熊果酸(31.5、62.5、125、250、500μg/ml)处理不同时间(12、24、48 h),MTT法检测细胞增殖抑制率。用125μg/ml熊果酸和100μmol/L PD98059(ERK抑制剂)处理HUVECs 48 h;不同浓度熊果酸处理24 h;125μg/ml熊果酸处理不同时间,RT-PCR和Western blot分别检测HUVECs中ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA的转录水平和蛋白的表达水平。结果不同浓度的熊果酸对HUVECs的增殖均有明显的抑制作用,且呈剂量和时间依赖性;经熊果酸和PD98059处理的HUVECs中ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA和蛋白的表达水平均明显降低,且呈剂量和时间依赖性。结论熊果酸可通过抑制ERK信号转导通路而抑制血管内皮细胞增殖。
Objective To investigate the effect of ursolic acid（UA） on the proliferation of human umbilical vein endothelial cells（HUVECs） and the expression of mRNAs and proteins of ERK1,c-Jun,c-Myc and Cyclin D1 genes as well as the mechanism of UA in inhibiting the growth of blood vessel.Methods HUVECs were cultured in vitro and treated with UA at various concentrations（31.5,62.5,125,250 and 500 μg/ml） for various hours（12,24 and 48 h）.The inhibiting rate of cell proliferation was determined by MTT method.HUVECs were treated with 125 μg/ml UA and 100 μmol / L PD98059（an ERK inhibitor） for 48 h respectively;with various concentrations of UA for 24 h;and with 125 μg/ml UA for various hours,in which the transcription levels of extracellular signal-regulated kinase（ERK） 1,c-Jun,c-Myc and Cyclin D1 mRNAs were determined by RT-PCR,and the expression levels of the corresponding proteins by Western blot.Results The UA at various concentrations showed significantly dose-and time-dependent inhibitory effect on the proliferation of HUVECs.Both the expression levels of mRNAs and proteins of ERK1,c-Jun,c-Myc and Cyclin D1 genes in HUVECs after treated with UA and PD98059 decreased significantly,in dose-and time-dependent modes.Conclusion UA inhibited the proliferation of HUVECs by inhibiting ERK signal transduction pathway.
出处
《中国生物制品学杂志》
CAS
CSCD
2011年第2期157-162,168,共7页
Chinese Journal of Biologicals
基金
重庆市自然科学基金(2006BB5298)
重庆市教委科技研究项目基金(KJ08032)
关键词
熊果酸
内皮细胞
细胞增殖
细胞外信号调节激酶信号转导通路
Ursolic acid（UA）
Endothelial cells
Cell proliferation
Extracellular signal-regulated kinase（ERK） signal transduction pathway
