摘要
目的观察消痰散结方对裸鼠MKN-45人胃癌组织中血管内皮生长因子C(vascular endothelialgrowth factor-C,VEGF-C)及血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)表达的影响,探讨消痰散结方防治胃癌淋巴转移的作用机制。方法 30只裸鼠建立MKN-45人胃癌原位移植模型,并随机分为生理盐水组、5-氟尿嘧啶(5-Fu)组及消痰散结方组,每组均为10只,分别给予生理盐水、5-Fu和消痰散结方干预后,运用免疫组织化学和RT-PCR方法检测瘤组织中微淋巴管密度(lymphaticmicro-vessel density,LMVD),VEGF-C及其受体VEGFR-3蛋白和mRNA的表达。结果消痰散结方组可以降低裸鼠移植瘤组织中LMVD,下调肿瘤组织中VEGF-C及其受体VEGFR-3蛋白和mRNA表达,与生理盐水组比较差异均有统计学意义(P<0.05,P<0.01);与5-Fu组比较差异均无统计学意义(P>0.05)。结论通过调控淋巴管生长因子VEGF-C及其受体VEGFR-3蛋白和mRNA的表达,可能是消痰散结方防治胃癌淋巴转移的机制之一。
Objective To observe the mechanism of Xiaotan Sanjie Recipe （XSR） in inhibiting lymphatic metastasis of gastric carcinoma by observing its effects on the expressions of vascular endothelial growth factor-C （VEGF-C） and its receptor VEGFR-3 in nude mice with transplanted human gastric adenocarcinoma cell MKN-45. Methods Thirty mice were made into MKN-45 tumor model and randomly divided into three groups treated with saline,5-Fu,and XSR,respectively. Gene and protein expressions of VEGF-C and VEGFR-3 in the tumor tissue were detected by RT-PCR,and the lymphatic microvessel density （LMVD） in tumor was measured with immunohistochemistry. Results Compared with the group treated with saline,the mRNA and protein expressions of VEGF-C and VEGFR-3 as well as LMVD level were significantly lower （P0.05 or P0.01） in the group treated with XSR. There was no significantly statistic difference between the group of XSR and 5-Fu on the indices mentioned above（P0.05）. Conclusion By down-regulaing the mRNA and protein expressions of VEGF-C and VEGFR-3 might be one of possible mechanisms for XSR in preventing and curing the lymphatic metastasis of gastric carcinoma.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2011年第2期204-208,共5页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
消痰散结方
胃肿瘤
血管内皮生长因子
血管内皮生长因子受体
转移
Xiaotan Sanjie Recipe
gastric carcinoma
vascular endothelial growth factor
vascular endothelial growth factor receptor
metastasis
