摘要
菌株Lj20是从健康辣椒植株分离得到的一株有抗真菌活性的植物内生放线菌,为了进一步明确其生物防治潜力,利用大孔吸附树脂、离心薄层层析以及柱层析等技术对Lj20代谢产物中的抗真菌活性物质进行了分离和提纯,并采用生长速率法测定了其代谢产物对黄瓜灰霉病菌的抑制作用,同时在温室中对Lj20发酵液粗提物的防病效果进行了试验。从Lj20代谢产物中分离得到一个抗真菌活性物质,该化合物对黄瓜灰霉病菌具有较强抑制作用,EC50值为174.94 mg/L;500mg/L Lj20发酵液粗提物对黄瓜灰霉病的防治效果达62.5%;经鉴定其化合物结构为3,5-二叔丁基-4-羟基-苄基甲醚。结果表明,放线菌Lj20有进一步开发和利用的价值。
Endophytic actinomycete Lj20 with antifungal activity was isolated from healthy capsicum plants.In order to determine its potential application in biological control,the antifungal activity substance was separated and purified from metabolites of Lj20 by the means of macroporous absorbing resin,centrifugal thin layer chromatography and column chromatography.The inhibitory efficacy of Lj20 metabolites on Botrytis cinerea Pers was also studied by means of the inhibition rate of mycelium growth in vitro and the control efficacy in the greenhouse.The results showed that the structure of a compound isolated from the Lj20 metabolites was identified as 3,5-di-tert-butyl-4-hydroxybenzyl methyl ether by analysis of IR（infrared spectroscopy） and 1H-NMR（proton nuclear magnetic resonance spectroscopy） spectroscopic data.This compound indicated the inhibition efficacy on B.cinerea in vitro with the EC50 was 174.94 mg/L.In addition,the control efficacy of crude extracts from Lj20 fermentation on B.cinerea reached 62.5% at the concentration of 500 mg/L in the greenhouse experiments,indicating that this endophytic actinomycete Lj20 had a potential value for further exploitation and utilization.
出处
《植物保护学报》
CAS
CSCD
北大核心
2011年第1期42-46,共5页
Journal of Plant Protection
基金
山西省科技攻关项目(20100311038
2007031039)
山西省留学基金项目(2009043)
