摘要
以贺兰山紫蘑菇为材料,采用纤维素酶酶法提取多糖,并经离子交换纤维素柱层析和葡聚糖凝胶柱层析对其组分进行分离和纯化,以明确贺兰山紫蘑菇的多糖组份和纯化方法。结果表明:贺兰山紫蘑菇多糖含有Ⅰ和Ⅱ2个组分,纯化后二组分均不合有核酸和蛋白质,此方法适宜该蘑菇多糖的分离纯化。
Taking Cortinarius rufo-olivaceus as tha test material,using method of cellulase enzyme extracted polysaccharides,and cellulose by ion exchange chrom a tography and sephadex column chromatography separation and purification of it＇s components,in order to determine the Cortinarius rufo-olivaceus polysaccharide components and purification methods.The results showed that there were two main components in polysaccharide,and the two components do not contain nuclear acid and protein after purification,the method was suitable for separation and purification of polysaccharides from Cortinarius rufo-olivaceus.
出处
《北方园艺》
CAS
北大核心
2011年第4期187-189,共3页
Northern Horticulture
基金
北方民族大学科研资助项目(2009Y040)
关键词
紫红丝膜菌
多糖
层析
纯化
Cortinarius rufo-olivaceus
polysaccharide
chromatography
purification
