摘要
以大岩桐叶为外植体,进行丛生芽和生根诱导试验,以期建立大岩桐产业化组培快繁的工艺流程和最佳培养方式。结果表明:丛生芽增殖最佳培养基为6-BA 1.5 mg/L+NAA0.05 mg/L,增殖倍数达6.67倍,在珍珠岩、蛭石、河砂组成混合的介质中完成诱导生根、壮苗和练苗,生根率达96%,移栽后成活率达90%以上。
Taking leaf of Sinningia speciosa as explants,the effect of different concentrations of hormones on induction of multiple shoots and rooting were studied.The results showed that best medium for proliferation of multiple shoots was 6- BA 1.5 mg/L＋NAA 0.05 mg/L,the proliferation of multiple was 6.67 times,a complete rooting,strong seedlings and training seedlings,the rooting rate was 96%,survival rate after transplanting more than 90%in perlite,vermiculite,sand was composed of mixed media.
出处
《北方园艺》
CAS
北大核心
2011年第4期153-154,共2页
Northern Horticulture
基金
科技部"十一五"国家科技支撑计划资助项目(2006BAI06A15-13)
关键词
大岩桐
组培快繁
二步法
产业化
Sinningia speciosa
rapid propagation
two steps way
industrial procedure
