摘要
目的 探讨组织蛋白酶D（cathepsin D）在鼻咽癌（nasopharyngeal carcinoma,NPC）中的表达及其临床病理意义.方法 采用免疫组织化学S-P法检测28例正常鼻咽黏膜上皮组织（normal nasopharyngal epithelial tissue,NNET）及72例NPC组织中cathepsin D蛋白的表达情况.结果 Cathepsin D在NPC中的表达较NNET明显下调（x2=13.55,P＜0.01）;Cathepsin D表达与NPC分化程度有关（P＜0.01）,在原发NPC中,与高分化（WHO type Ⅰ）和中度分化（WHO type Ⅱ）NPC相比,cathepsin D蛋白在低未分化（WHO type Ⅲ）NPC中.的表达明显下调（x2=41.47,P＜0.01）;Cathepsin D的表达水平与NPC临床分期、复发、局部淋巴结和远处转移有关（x2=13.43,22.86,21.61,14.32,P＜0.01）,cathepsin D高表达的肿瘤较低表达的肿瘤有更晚的临床分期、更容易复发、更容易发生局部淋巴结和远处转移（P＜0.05或P＜0.01）.结论 Cathepsin D的表达水平与NPC的分化、临床分期、病理分级、复发及转移有关,有望成为NPC分化的分子标志物及潜在的治疗靶标.
Objective To evaluate the expression level of cathepsin D protein in nasopharyngeal carcinoma （NPC） and to determine its relationship with clinic pathological characters. Method Immunohistochemistry was performed to detect the expression of cathepsin D in 72 cases of primary NPC and 28 cases of normal nasopharyngal epithelial tissue （ NNET）, and the correlation of its expression level with clinicopathologic features and clinical outcomes was evaluated. Results Significant down-regulation of cathepsin D was observed in NPC versus NNET（x2 = 13. 55, P ＜0. 01 ）. In addition, cathepsin D down-regulation was significantly correlated with poor histological differentiation （ x2 =41.47, P ＜0. 01 ）. Type Ⅰ and Ⅱ （well-moderately differentiated） NPC showed more intense immunoreactivity of cathepsin D compared with type Ⅲ （poorly differentiated） NPC. Cathepsin D up-regulation was significantly correlated with clinical stage, recurrence, and lymph node and distant metastasis （x2 = 13.43,22. 86,21.61,14. 32, P ＜0. 01 ）.Tumors with cathepsin D up-regulation tended to have advanced clinical stage, frequent recurrences, and metastasis to lymph node and distant organ. Conclusion The expression of cathepsin D was closely related with the differentiation, clinical stage and pathological grade of NPC. Cathepsin D could be served as an effective differentiation marker for the histopathological grading of NPC and a possible therapy target.
出处
《中国医师杂志》
CAS
2010年第12期1590-1593,共4页
Journal of Chinese Physician
基金
国家自然科学基金（81072198）
湖南省教育厅重点项目（10A104）
湖南省自然科学基金（10JJ6035）
