摘要
目的探讨本地区汉族人群线粒体DNA ND1基因中T3394C突变与2型糖尿病(T2DM)的关系。方法应用聚合酶链反应(PCR)对无血缘关系的225例T2DM患者和190名正常对照者的外周血DNA进行PCR扩增并直接测序确证。结果糖尿病患者组中检出T3394C突变8例(3.56%),正常对照组检出1名(0.53%),2组间比较差异有统计学意义(P<0.05)。结论线粒体DNA T3394C突变可能与本地区汉族人群T2DM的发生有关。
Objective To study the relationship of T3394C point mutation in the mitochondrial DNA ND1 gene with type 2 diabetes mellitus（T2DM） in local Han people.Methods The peripheral blood DNA was amplified and sequenced in 225 T2DM patients and 190 controls by polymerase chain reaction（PCR）.Results There were 8 cases（3.56%）of T3394C mutation in T2DM patients and 1 case（0.53%）in controls.There was significant difference between the 2 groups（P0.05）.Conclusions The point mutation of T3394C in mitochondrial DNA could be related to the occurrence of T2DM in local Han people.
出处
《检验医学》
CAS
北大核心
2011年第2期122-124,共3页
Laboratory Medicine
关键词
线粒体ND1基因
2型糖尿病
基因突变
Mitochondrial DNA ND1 gene
Type 2 diabetes mellitus
Gene mutation
