摘要
目的：探讨细胞凋亡与急性肺损伤（ＡＬＩ）发生、发展的关系以及Ｆａｓ和／ＦａｓＬ系统表达改变的意义。方法：复制内毒素性大鼠ＡＬＩ模型；采用ＴＵＮＥＬ法、原位杂交、半定量逆转录聚合酶链反应（ＳｑＲＴ ＰＣＲ）及免疫组化等技术观察大鼠ＡＬＩ发生过程中，肺组织细胞凋亡变化以及Ｆａｓ／ＦａｓＬ系统蛋白质和ｍ ＲＮＡ表达的改变。结果：内毒素性ＡＬＩ早期（＜ ２４ 小时），大鼠肺泡上皮细胞和肺血管内皮细胞凋亡明显增加；肺组织Ｆａｓ／ＦａｓＬｍ ＲＮＡ和蛋白质表达明显上调，且与肺组织细胞凋亡的增加相一致。结论：肺组织细胞凋亡以及Ｆａｓ／ＦａｓＬ系统表达明显上调可能参与大鼠ＡＬＩ的发病机制。
Objective:To determine the potential role of apoptosis and Fas/Fas ligand system expression in the pathogenesis of acute lung injury (ALI).Methods:Using TUNEL,in situ hybridization,reversetranscription polymerase chain reaction (RTPCR),and immunocytochemistry techniques,pulmonary apoptosis, Fas/FasL expressions were detected in rats during early phase of ALI.Results:Endotoxin challenge resulted in the rapid appearance of apoptosis in alveolar epithelial cells and pulmonary vascular endothelial cells at early stage of ALI.The expression of Fas/FasL mRNA and protein was upregulated in lung tissues of rats with ALI ( P <0 01 ),which was related to the elevation of pulmonary apoptosis.Conclusions:These results suggest that the excessive apoptosis and Fas/FasL system expression may play a role in the pathogenesis of endotoxininduced ALI in rats.
出处
《中国危重病急救医学》
CSCD
1999年第11期662-665,共4页
Chinese Critical Care Medicine
关键词
肺损伤
急性
内毒素
细胞凋亡
基因表达
acute lung injury
endotoxin
apoptosis
gene expression
Fas/FasL
