摘要
目的:探讨吐温80作为Am es试验中样品溶剂的可行性。方法:分别以二甲基亚砜和含25%吐温80的水溶液为溶剂,进行蜂胶类、鱼油类、辅酶Q10类产品的溶解度试验和Am es试验。结果:蜂胶类、鱼油类、辅酶Q10类产品在二甲基亚砜中的溶解度分别为在含25%吐温80的水溶液中溶解度的23.30%、37.26%和26.73%。两种溶剂对TA97、TA98、TA100和TA102菌株均无毒性;同一样品5个剂量组在不同溶剂条件下的实验结果均无显著性差别;三种样品的Am es试验结果均为阴性。结论:吐温80是一种较为理想的Am es试验样品溶剂。
Objective：To investigate the feasibility of Tween 80 used as a sample solvent in Ames test.Methods： Dimethyl sulfoxide（DMSO） and 25% Tween 80 aqueous solution were used to solve propolis,fish oil and coenzyme Q10 to make solubility tests and Ames tests.Results： The solubilities of propolis,fish oil and coenzyme Q10 in DMSO were only 23.30%,37.26% and 26.73% of those in 25% Tween 80 aqueous solution.Both solvents were nontoxic for TA97,TA98,TA100 and TA102 Salmonella bacteria strains.In all 5 dosage groups,the Ames test results for every sample solved in 25% Tween 80 aqueous solution were the same as those solved in DMSO.The Ames test results of 3 samples were all negative.Conclusion： Tween 80 is an ideal sample solvent in Ames test.
出处
《中国卫生检验杂志》
CAS
2011年第2期371-374,共4页
Chinese Journal of Health Laboratory Technology
