摘要
目的:建立土茯苓总黄酮的含量测定方法。方法:采用70%乙醇超声提取工艺和可见分光光度法测定土茯苓中总黄酮的含量,以芦丁为对照品,在500 nm波长处测量。结果:芦丁在0.008～0.048 mg.mL-1范围内线性关系良好(r=1.000),平均加样回收率(n=6)以芦丁计为98.34%,RSD为3.4%。结论:此方法操作简单,精密度、稳定性和重复性良好,可用于土茯苓总黄酮含量测定。
Objective： To explore the determination method of total flavonoids in Rhizoma Smilacis Glabrae.Method： Total flavonoids in Rhizoma Smilacis Glabrae was determined by visible spectrophotometry.Lutin was used as reference substance,and the detection wavelength was set at 500 nm.An ultrasonic extraction process with 70% alcohol was adopted.Results： The linear ranges of Lutin was 0.008-0.048 mg·mL-1（r=1.000）.The average recoveries（n=6）were 98.34% and the RSD was 3.4%.Conclusion： The visible spectrophotometry method is reliable.It could be used to determine the content of total flavonoids in Rhizoma smilax glabrae to control its quantity in the phamaceutical preparations.
出处
《现代生物医学进展》
CAS
2011年第1期131-133,共3页
Progress in Modern Biomedicine
基金
国家自然科学青年基金(NO.81001647)
浙江省科技厅面上项目(2009C33005)
中国博士后科学基金面上项目(20100471757)
关键词
土茯苓
总黄酮
含量测定
可见分光光度法
Rhizoma Smilacis Glabrae
total flavonoids
determination
VIS spectortrophotography.
