摘要
目的:探讨喉癌中Mdm2、ToPoⅡ蛋白和ToPoⅡmRNA的表达及与临床病理特征的关系。方法:采用免疫组化S-P法检测Mdm2、ToPoⅡ蛋白在50例喉鳞状细胞癌和10例喉正常黏膜中的表达,采用原位杂交方法检测相应标本中ToPoⅡmRNA的表达。结果:(1)Mdm2在喉癌和喉正常组织中表达的阳性率分别是86%(43/50)和20%(2/10),Mdm2与临床分期和淋巴结转移有关,与性别、分化程度、临床分型无关。(2)ToPoⅡ蛋白和ToPoⅡmRNA在喉癌表达的阳性率分别是70%(35/50)和64%(32/50),在正常组织中表达的阳性率分别是10%(1/10)和20%(2/10),ToPoⅡ与淋巴结转移、临床分期和分化程有关,ToPoⅡ与临床化疗关系密切,与性别、临床分型无关。ToPoⅡ蛋白和ToPoⅡmRNA在喉癌组织的阳性表达呈正相关(P<0.05)。(3)二者相关性:本研究得出Mdm2与ToPoⅡ呈负相关(r=-0.946)。结论:Mdm2、ToPoⅡ的异常表达可能对喉癌的发生发展起着不同作用,通过对它们的检测可以对喉癌的预后及化疗提供理论依据。
Objective：To investigate the relationship between the proteinum expression of Mdm2,Topo Ⅱ and Topo Ⅱ gene mRNA in laryngeal carcinoma,and their relationship with clinic opathological feature. Methods： Expression of Mdm2and ToPo Ⅱ protein was measured with immunohistochemistry method in 50 cases of laryngeal squamous cell carcinoma and 10 cases of normal laryngeal mucosa,and the expression of Topo II mRNA was detected by insitu hybridization in conesponding samples.Results：（1）The expression of Mdm2 positive was 86%（43/50）, 20%（2/10）in laryngeal carcinoma and normal laryngeal mucosa.Mdm2 expression level was correlated with clinical stages and lymphnode metastasis,and had no correlation with sex, differentiation level, clinical stages and T stages.（2）The positive ratio expression of Topo Ⅱ protein and gent mRNA in laryngeal carcinoma was 70%（35/50）and 64%（32/50）,and that in normal laryngeal mucosa was 10%（1/10） and 20%（2/10）.Topo Ⅱ expression level was correlated with clinical stages,lymphnode metastasis and differentiation level. Topo Ⅱ related intimating with clinic chems.And had no relation with sex, clinical classification and T stages. The expression of TopoⅡ gene mRNA and protein had consistency （P〈0.05）.（3）The three agent dependablity：The study showing Mdma2 and Topo II had negative correlation（r=-0.946）,Mdma2 and Topo II had no obviously dependablity.Conclusinn： This experiment suggested that Mdm2 and ToPo I1 may play certain roles in the oncogengsis and progession of laryngeal carcinoma.Detection of these gene products had positive offects on early diagnosis,malignancy determination prognosis and treatment of ehems laryngeal carcinoma.
出处
《中国医药导刊》
2011年第1期112-114,共3页
Chinese Journal of Medicinal Guide
